Article

In vitro characteristics of an Atlantic salmon (Salmo salar L.) hind gut microbial community in relation to different dietary treatments

Details

Citation

Zarkasi KZ, Taylor RS, Glencross B, Abell GCJ, Tamplin ML & Bowman JP (2017) In vitro characteristics of an Atlantic salmon (Salmo salar L.) hind gut microbial community in relation to different dietary treatments. Research in Microbiology, 168 (8), pp. 751-759. https://doi.org/10.1016/j.resmic.2017.07.003

Abstract
In this study, microbial community dynamics were assessed within a simple invitro model system in order to understand those changes influenced by diet. The abundance and diversity of bacteria were monitored within different treatment slurries inoculated with salmon faecal samples in order to mimic the effects of dietary variables. A total of five complete diets and two ingredients (plant meal) were tested. The total viable counts (TVCs) and sequencing data revealed that there was very clear separation between the complete diets and the plant meal treatments, suggesting a dynamic response by the allochthonous bacteria to the treatments. Automated ribosomal intergenic spacer analysis (ARISA) results showed that different diet formulations produced different patterns of fragments, with no separation between the complete diets. However, plant-based protein ingredients were clearly separated from the other treatments. 16S rRNA Illumina-based sequencing analysis showed that members of the genera Aliivibrio,Vibrio and Photobacterium became predominant for all complete diets treatments. The plant-based protein ingredient treatments only sustained weak growth of the genusSphingomonas. Invitro based testing of diets could be a useful strategy to determine the potential impact of either complete feeds or ingredients on major fish gastrointestinal tract microbiome members.

Keywords
Intestinal bacteria; In vitro model system; 16S rRNA gene; Dietary treatments; Atlantic salmon

Journal
Research in Microbiology: Volume 168, Issue 8

StatusPublished
Publication date31/10/2017
Publication date online17/07/2017
Date accepted by journal10/07/2017
URLhttp://hdl.handle.net/1893/25845
PublisherElsevier
ISSN0923-2508

People (1)

People

Professor Brett Glencross
Professor Brett Glencross

Honorary Professor, Institute of Aquaculture