Stirling virologist at front line of research into Ebola epidemic

Scientist behind 15-minute detection test takes on new project

Manfred Weidmann
Virologist Dr Manfred Weidmann

A University of Stirling scientist is at the centre of ground-breaking research to tackle the Ebola epidemic.

Virologist Dr Manfred Weidmann is the only British University scientist involved in a new international collaboration focused on developing a biosafe point-of-care detection method for immediate use in fighting Ebola-hit West Africa.

Dr Weidmann played a major role in the development of an Ebola rapid detection and diagnosis test, which is currently being used in Guinea in a project funded by the Wellcome Trust and led by the Pasteur Institute in Dakar, Senegal.

Now he is adapting this into a paper strip method – similar to a pregnancy test - which could provide a simple, cost-effective diagnosis method for the deadly disease.

The two-year Innovative Medicines Initiative (IMI) is part-funded by the European Union and led by the Swedish Public Health Agency. It brings together scientists from across Europe and Africa to develop bedside rapid diagnostics for Ebola. Dr Weidmann will be working closely with colleagues in Public Health England.

Dr Weidmann, from the University’s School of Natural Sciences, said: “If you use a strip-test then you don’t need a complicated detection device and this could then be developed into a bedside test. In the particular case of Ebola, however, the bedside test would most likely be a test in an adjacent laboratory as it is vital you take all steps to avoid contamination.

“There are similar strip-tests under development elsewhere, but the method we are working on is the most reliable as it ensures better sensitivity of detection.”

Ebola is diagnosed by locating the virus's genetic material in the blood of a patient, but this requires laboratories with sophisticated devices working at three different temperatures. Using a method called Recombinase Polymerase Amplification (RPA), Dr Weidmann created tests which work at one temperature and can detect a range of Filoviruses, thread-like viruses causing hemorrhagic fever, of which Ebola is one.

Working with researchers at the Pasteur Institute in Dakar, Senegal; the German Primate Centre; the Robert Koch Institute in Germany and Biocompany TwistDx, this method has been developed into a sophisticated point-of-care blood and saliva test, all contained within a solar-powered, mobile, suitcase-sized laboratory.

Following a successful pilot in Conakry, Guinea, two teams have now been trained and deployed, with a further three teams expected to join them in the coming months.

“Our test can go to the limit of detection in just six minutes,” added Dr Weidmann. “But the speed it takes isn’t the only point. Ebola is so infectious that you have to use glove boxes. Most of these are based in makeshift tented laboratories attached to hospitals. Our mobile lab is just that, totally mobile. It includes the glove box and you can take it to any location, set it up in half an hour and then start testing.

“I’m very proud of what we have been able to achieve so far, especially the fact we are the first research team to have empowered and trained local teams to conduct the on-site tests for themselves.

“The race is on to tackle the epidemic and my research is as much about developing diagnostic tests for the future. There are signs the epidemic is slowing although there remains lots of activity in Sierra Leone and Guinea. It is impossible to know how long it will continue as it’s all about human contact and if you can’t stop that then you can’t prevent the disease.”

David Tripp
Communications
01786 466687
david.tripp@stir.ac.uk

Background information

The rapid detection test project is funded jointly by the Department for International Development (DFID) and the Wellcome Trust, through its Research for Health in Humanitarian Crises (R2HC) initiative.

The paper strip adaptation is funded by the Innovative Medicines Initiative.

The University of Stirling is ranked fifth in Scotland and 40th in the UK for research intensity in the 2014 Research Excellence Framework. Stirling is committed to carrying out research which has a positive impact on communities across the globe – addressing real issues, providing solutions and helping to shape society. Interdisciplinary in its approach, Stirling’s research informs its teaching curriculum and facilitates opportunities for knowledge exchange and collaboration between staff, students, industry partners and the wider community. At almost 50-years-young, Stirling retains a pioneering spirit and a passion for innovation. Its scenic central Scotland campus – complete with a loch, castle and golf course – is home to more than 11,000 students and 1400 staff representing 115 nationalities. This includes an ever-expanding base for postgraduate study.

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