Safe limits of selenomethionine and selenite supplementation to plant-based Atlantic salmon feeds



Berntssen MHG, Betancor M, Caballero MJ, Hillestad M, Rasinger J, Hamre K, Sele V, Amlund H & Ørnsrud R (2018) Safe limits of selenomethionine and selenite supplementation to plant-based Atlantic salmon feeds. Aquaculture, 495, pp. 617-630.

The use of plant-based feeds warrants the supplementation with selenium (Se) to cover the requirement for Atlantic salmon. Depending on its chemical form, Se is a trace element with a narrow range between requirement and toxicity for most vertebrates. Information on safe upper limit for Atlantic salmon feed supplementation is lacking. Atlantic salmon (147 g) were fed a low natural background organic Se diet (0.45 mg Se kg−1, wet weight (ww)) fortified with 5 graded levels of inorganic sodium selenite (0.45, 5.4, 11.0, 29.4, or 60.0 mg kg−1 ww) or organic selenomethionine (SeMet) (0.45, 6.2, 16.2, 21, or 39 mg kg−1 ww), in triplicate for 3 months. Excess Se supplementation was assessed by targeted biomarkers of Se toxicity pathways (e.g. markers of oxidative stress and lipid metabolism), as well as general adverse effect parameters (plasma biochemistry, hematology, liver histopathology, and growth). Safe limits were set by model-fitting the effect data in a dose-response (lower bound) bench mark dose (BMDL) evaluation. Fish fed the two highest selenite levels showed mortality while fish fed SeMet had no mortality. Fish fed 5.4–11 mg selenite kg−1 feed showed significantly (ANOVA, Tukey's t-test, p

Atlantic salmon; Selenite; Selenomethionine; Toxicity; Bench mark dose

Aquaculture: Volume 495

FundersFiskeri - og havbruksnæringens forskningsfond, Skretting AS, Biomar AS, Marine Harvest ASA and Cargill Aqua Nutrition AS
Publication date01/10/2018
Publication date online19/06/2018
Date accepted by journal16/06/2018
PublisherElsevier BV

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Dr Monica Betancor
Dr Monica Betancor

Associate Professor, Institute of Aquaculture