Citation Vallejo-Marin M, Solis-Montero L, Bacles C & Lepais O (2011) Thirteen microsatellites developed by SSR-enriched pyrosequencing for Solanum rostratum (Solanaceae) and related species. American Journal of Botany, 98 (10), pp. e296-e299. https://doi.org/10.3732/ajb.1100159
Abstract Premise of the study: Microsatellite markers were developed using second-generation sequencing in Solanum rostratum as a tool to study the reproductive biology and genetic structure of this invasive species.
Methods and Results: Thirteen microsatellites were successfully discovered and amplified in a single multiplexed PCR. All loci showed genetic variation in S. rostratum. Cross-amplification in five closely related taxa was successful for a subset of loci.
Conclusions: The set of 13 microsatellite markers developed here provides a time-effective and cost-effective genetic tool to study the reproductive biology of S. rostratum. The demonstrated transferability of the PCR multiplex to related taxa also highlights its usefulness for evolutionary studies across Solanum sect. Androceras.
Keywords invasive species; population genetics; reproductive biology; Solanum rostratum; Solanum fructu-tecto; Solanum heterodoxum; Solanum grayi var. grayi; Solanum grayi var. grandiflorum; Solanum lumholtzianum; Solanum sect. Androceras
Journal American Journal of Botany: Volume 98, Issue 10