Intrafamilial Genotyping of Helicobacter pylori from Faecal DNA

Citation

McMillan M, Mackay WG, Williams CL, Shepherd A, Malcolm C & Weaver LT (2011) Intrafamilial Genotyping of Helicobacter pylori from Faecal DNA. Gastroenterology Research and Practice, 2011 (Article ID 491035), pp. 1-7. https://doi.org/10.1155/2011/491035

Abstract
Helicobacter pylori infection, often acquired in early childhood, is a global cause of undernutrition, gastritis, peptic ulcer disease and gastric carcinoma. This study tested the feasibility of using H. pylori shed in the faeces as a source of DNA for non-invasive epidemiological studies. H. pylori DNA was chemically recovered and isolated using a specific biotinylated oligonucleotide probe with magnetic capture from 28 H. pylori positive faecal samples obtained from children attending hospital for the investigation of suspected H. pylori infection, together with close family members. Random amplification of polymorphic DNA (RAPD) was subsequently used to discriminate each isolate. 93% of stool samples selected were typeable. Parent, child and sibling samples were compared and similarities determined. Phylogenetic analysis showed that H. pylori DNA obtained from the faeces can be used to genotype individual strains, offering a means of studying intrafamilial transfer of this microorganism.

Keywords
helicobacter pylori; Helicobacter pylori infections; DNA Analysis

Journal
Gastroenterology Research and Practice: Volume 2011, Issue Article ID 491035

People

Professor Ashley Shepherd

Professor, Health Sciences Stirling