Identification of B-cell epitopes on the betanodavirus capsid protein


Costa JZ, Adams A, Bron J, Thompson K, Starkey W & Richards R (2007) Identification of B-cell epitopes on the betanodavirus capsid protein. Journal of Fish Diseases, 30 (7), pp. 419-426.

The pepscan procedure was used to identify betanodavirus B-cell epitopes recognized by neutralizing mouse monoclonal antibodies (MAbs) and serum samples obtained from sea bass, Dicentrarchus labrax, naturally infected with betanodavirus. Pepscan was performed with a panel of thirty-four 12-mer synthetic peptides that mimicked the entire betanodavirus capsid protein. Sea bass serum samples reacted strongly with three regions of the capsid protein comprising amino acid residues 1-32, 91-162 and 181-212. The latter region was also recognized by neutralizing MAbs and coincided with a region of high antigenic propensity identified by an antigen prediction algorithm. These data suggest that a region of the betanodavirus capsid protein spanning amino acid residues 181-212 may represent a neutralization domain that could potentially be used to inform the development of nodavirus vaccines and immunodiagnostic reagents

algorithm; ANTIBODY; ANTIGEN; AQUACULTURE; Atlantic cod; BASS; betanodavirus; COAT PROTEIN; data; Development; Dicentrarchus labrax; DICENTRARCHUS-LABRAX; Disease; DISEASES; epitope mapping; fish; GROUPER; Health; identification; INFECTION; luminex; MASS MORTALITIES; monoclonal antibodies; monoclonal antibody; NODAVIRUS; pepscan; Prediction; PROTEIN; REGION; regions; SAMPLE; SAMPLES; SEA; SEA BASS; SEA-BASS; SERUM; SYNTHETIC PEPTIDES; VACCINE; VACCINES; VIRAL NERVOUS NECROSIS; VIRUS; welfare; xMAP technology

Journal of Fish Diseases: Volume 30, Issue 7

Publication date31/07/2007
Publication date online20/06/2007