Citation Ross L, Sanchez Blanco J, Martinez-Palacios CA, Racotta IS & Toledo Cuevas M (2007) Anaesthesia, sedation and transportation of juvenile Menidia estor (Jordan) using benzocaine and hypothermia. Aquaculture Research, 38 (9), pp. 909-917. https://doi.org/10.1111/j.1365-2109.2006.01642.x
Abstract Anaesthesia, sedation and transportation of the Atherinopsid, Menidia estor, were investigated using benzocaine, hypothermia and combinations of the two. The optimum dose rates of benzocaine for sedation of juvenile Chirostoma estor were 15 and 18 mg L-1. When hypothermia was used alone, stable sedation of C. estor was induced at 15 and 12 degrees C, with no mortalities and full recovery after about 8 min. There was synergism when benzocaine and hypothermia were combined, with the optimal combination being at 15 degrees C and 12 mg L-1 benzocaine. Successful transportation was conducted over 3.5 and 8.5 h using combined benzocaine and hypothermia. Aloe vera extract used in the transportation medium reduced scale loss. This work has, for the first time, defined a systematically derived system of safe, long distance, live transportation of this species and others of the genus Menidia.