Interferon type I and type II responses in an Atlantic salmon (Salmo salar) SHK-1 cell line by the salmon TRAITS/SGP microarray

Citation

Martin SAM, Taggart J, Seear PJ, Bron J, Talbot RT, Teale AJ, Sweeney GE, Hoyheim B, Houlihan DF, Tocher DR, Zou J & Secombes CJ (2007) Interferon type I and type II responses in an Atlantic salmon (Salmo salar) SHK-1 cell line by the salmon TRAITS/SGP microarray. Physiological Genomics, 32 (1), pp. 33-44. https://doi.org/10.1152/physiolgenomics.00064.2007

Abstract
Interferons (IFNs) are cytokines that have proinflammatory, antiviral, and immunomodulatory effects and play a central role during a host response to pathogens. The IFN family contains both type I and type II molecules. While there are a number of type I IFNs, there is only one type II IFN. Recently both type I and type II IFN genes have been cloned in salmonid fish and recombinant proteins produced showing IFN activity. We have stimulated an Atlantic salmon cell line (SHK-1) with both type I and type II recombinant salmonid IFNs and analyzed the transcriptional response by microarray analysis. Cells were exposed to recombinant IFNs for 6 or 24 h or left unexposed as controls. RNA was hybridized to an Atlantic salmon cDNA microarray (salmon 17K feature TRAITS/SGP array) in order to assess differential gene expression in response to IFN exposure. For IFN I and II, 47 and 72 genes were stimulated, respectively; most genes were stimulated by a single IFN type, but some were affected by both IFNs, indicating coregulation of the IFN response in fish. Real-time PCR analysis was employed to confirm the microarray results for selected differentially expressed genes in both a cell line and primary leukocyte cultures

Keywords
activities; analysis; ATLANTIC; ATLANTIC salmon; BIOLOGY; CDNA MICROARRAY; CELLS; Control; CULTURE; CXC CHEMOKINE; DNA VACCINATION; DOUBLE-STRANDED-RNA; exposure; expression; EXPRESSION ANALYSIS; families; FAMILY; Feature; fish; FUGU-RUBRIPES; gene; GENE expression; GENE-EXPRESSION; Genes; GENETICS; Health; HOST; immune; microarray; microarray analysis; MOLECULAR-CLONING; NECROSIS VIRUS; NUMBER; ORDER; PATHOGEN; PATHOGENS; PCR; Play; primary; PROTEIN; RAINBOW-TROUT; responses; RNA; Role; SALAR; Salmo salar; SALMO-SALAR; Salmon; Scotland; SINGLE; transcriptome; TROUT ONCORHYNCHUS-MYKISS; welfare

Journal
Physiological Genomics: Volume 32, Issue 1

People

Professor James Bron

Professor, Institute of Aquaculture