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Article

A piscine glutathione S-transferase which efficiently conjugates the end-products of lipid peroxidation

Citation
Leaver M & George S (1998) A piscine glutathione S-transferase which efficiently conjugates the end-products of lipid peroxidation. Marine Environmental Research, 46 (1-5), pp. 71-74. http://www.sciencedirect.com/science/article/pii/S0141113697000718; https://doi.org/10.1016/S0141-1136%2897%2900071-8

Abstract
A cDNA clone for glutathione S-transferaseA (GSTA) from plaice (Pleuronectes platessa) was expressed in Eschericia coli (E. coli) and purified to homogeneity by S-hexylglutathione affinity chromatography. When compared to literature values for a variety of purified mammalian GSTs, the heterologously expressed purified plaice enzyme had moderate activity towards the model substrate 1,2-chloro-2,4-dinitrobenzene (CDNB) and exhibited a Km of 2.5 ± 2 mM and Vmax of 30.9 ± 2.3 μmol min-1 mg-1. It had little or no activity towards several other model GST substrates including 1,2-dinitrochloro-4-benzene (DCNB), ethacrynic acid (EA), and p-nitrobenzylchloride (NBC). However plaice GSTA was a relatively efficient catalyst for the conjugation of a series of alk-2-enals and alk-2,4-dienals and also 4-hydroxynonenal. The highest activity observed with this series of substrates was with trans-non-2-enal with a Km of 17.9 ± 2.2 μM and a Vmax of 3.01 ± 0.57 μmol min-1 mg-1. These unsaturated alkenals have been identified in cells and cell extracts as highly toxic products arising from peroxidation of unsaturated fatty acids particularly during periods of oxidative stress. Fish are relatively rich in polyunsaturated fatty acids and thus GSTA mediated conjugation may be an important mechanism for detoxifying peroxidised lipid breakdown products.

Journal
Marine Environmental Research: Volume 46, Issue 1-5

StatusPublished
Author(s)Leaver, Michael; George, Stephen
Publication date31/07/1998
Publication date online28/06/1999
URLhttp://hdl.handle.net/1893/7631
PublisherElsevier
Publisher URLhttp://www.sciencedirect.com/…0141113697000718
ISSN0141-1136
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