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Article

Evidence from heterologous expression of glutathione S-transferases A and A1 of the plaice (Pleuronectes platessa) that their endogenous role is in detoxification of lipid peroxidation products

Citation
Martinez-Lara E, Leaver M & George S (2002) Evidence from heterologous expression of glutathione S-transferases A and A1 of the plaice (Pleuronectes platessa) that their endogenous role is in detoxification of lipid peroxidation products. Marine Environmental Research, 54 (3-5), pp. 263-266. http://www.sciencedirect.com/science/article/pii/S0141113602001885; https://doi.org/10.1016/S0141-1136%2802%2900188-5

Abstract
cDNA clones for glutathione S-transferases A (GST-A) and A1 (GST-A1) from plaice (Pleuronectes platessa) were expressed as N-terminally 6XHis tagged proteins in Escherichia coli and purified to homogeneity from Ni-NTA silica. GST-A was an efficient catalyst for conjugation of unsaturated alkenals derived from peroxidation of polyunsaturated fatty acids with the highest activity observed with trans-non-2-enal (8 μmol min-1 mg-1). GST-A1 was a very efficient Se-independent glutathione peroxidase with an activity towards cumene hydroperoxide of 25 μmol min-1 mg-1 . Although the enzymes exhibited moderately high activities towards the model substrate 1-chloro-2,4-dinitrobenzene (CDNB) they exhibited little or no activity towards other common prototypical xenobiotic substrates. Together with data for ontogeny, tissue distribution and inducibility of these enzymes, we contend that a primary function of these enzymes is protection from the harmful effects of lipid peroxidation products generated naturally or exacerbated by xenobiotic exposure.

Keywords
plaice; glutathione-S-transferase; lipid oxidation

Journal
Marine Environmental Research: Volume 54, Issue 3-5

StatusPublished
Author(s)Martinez-Lara, Esther; Leaver, Michael; George, Stephen
Publication date30/09/2002
Publication date online28/05/2002
URLhttp://hdl.handle.net/1893/7615
PublisherElsevier
Publisher URLhttp://www.sciencedirect.com/…0141113602001885
ISSN0141-1136
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