Cloning of the major 3-MC inducible phase I and phase II detoxification enzymes of plaice liver

Citation

Pirrit LA, Healey R, Assheton H, Leaver M & George S (1995) Cloning of the major 3-MC inducible phase I and phase II detoxification enzymes of plaice liver. Marine Environmental Research, 39 (1-4), pp. 15-19. http://www.sciencedirect.com/science/article/pii/014111369400065W; https://doi.org/10.1016/0141-1136%2894%2900065-W

Abstract
cDNA's coding for cytochrome P4501A1 (CYP1A1), phenol UDP-glucuronosyltransferase (UDPGT) and glutathione S-transferase (GST-A) were cloned and sequenced from an expression library prepared from the liver of a 3-methylcholanthrene (3-MC) induced plaice. Plaice CYP1A1 and Phenol UDPGT display a high degree of structural conservation with homologous mammalian isoforms and their mRNAs were shown to be highly induced in liver after 3-MC treatment of fish. Expression of plaice GST-A, which displays closer homology to GSTs from plants and invertebrates than those of mammals, is repressed after 3-MC treatment.

Journal
Marine Environmental Research: Volume 39, Issue 1-4