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Article

Molecular cloning of troponin I expressed in fast white muscle of a teleost fish, the Atlantic herring (Clupea harengus L)

Citation
Hodgson P, Leaver M, George S, Maclean D & Hastings KEM (1996) Molecular cloning of troponin I expressed in fast white muscle of a teleost fish, the Atlantic herring (Clupea harengus L). Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1306 (2-3), pp. 142-146. http://www.sciencedirect.com/science/article/pii/0167478196000334; https://doi.org/10.1016/0167-4781%2896%2900033-4

Abstract
By cDNA cloning and 5′-RACE analysis we characterised a Clupea harengus troponin I mRNA expressed in larvae and in adult white (fast) muscle, but not in red (slow) or cardiac muscle. The mRNA encodes a TnI protein of the short chain length (176 residues) N-terminally truncated type previously observed only in tetrapod skeletal muscles. Despite its expression specificity the herring TnI does not particularly resemble the tetrapod fast skeletal muscle isoform in sequence but appears to be outside of the tetrapod TnIfast/TnIslow/TnIcardiac isoform family. Surprisingly, the actin/TnC-binding sequence resembles that of arthropod, rather than tetrapod vertebrate, troponin I's and has, besides, unique features not seen in any other troponin I's, vertebrate or invertebrate.

Keywords
troponin I; muscle protein; fish; molecular sequence data; evolution; vertebrate; (Clupea harengus)

Journal
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression: Volume 1306, Issue 2-3

StatusPublished
Author(s)Hodgson, Paul; Leaver, Michael; George, Stephen; Maclean, David; Hastings, Kenneth E M
Publication date02/05/1996
Publication date online02/03/1999
URLhttp://hdl.handle.net/1893/7726
PublisherElsevier
Publisher URLhttp://www.sciencedirect.com/…0167478196000334
ISSN0167-4781
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