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Article

Expression and tissue distribution of plaice glutathione S-transferase-A

Citation
Leaver M, Scott K & George S (1992) Expression and tissue distribution of plaice glutathione S-transferase-A. Marine Environmental Research, 34 (1-4), pp. 237-241. http://www.sciencedirect.com/science/article/pii/0141113692901142; https://doi.org/10.1016/0141-1136%2892%2990114-2

Abstract
Glutathione S-transferase (GST) activity was present in all tissues examined of a marine flatfish, the plaice (Pleuronectesplatessa). It was predominately expressed in liver, with lower but significant activities present in the kidney, intestine and gills. Immunoblot analysis with specific antisera to GST-A and B, the two major hepatic isoforms, showed that both were expressed in all tissues except spleen; however, clear differences in the relative proportions of total GST activity and these isoforms were observed. The expression of GST-A mRNA was also investigated by a cRNA probe and followed the enzyme protein levels. The results indicate that GST-A is the predominant isoform in gills and that other, so far uncharacterised isoforms, must account for the high enzyme activity measured by conjugation of chlorodinitrobenzene in liver.

Journal
Marine Environmental Research: Volume 34, Issue 1-4

StatusPublished
Author(s)Leaver, Michael; Scott, Karen; George, Stephen
Publication date31/12/1992
Publication date online15/04/2003
PublisherElsevier
Publisher URLhttp://www.sciencedirect.com/…0141113692901142
ISSN0141-1136
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