Citation Leaver M, Scott K & George S (1992) Expression and tissue distribution of plaice glutathione S-transferase-A. Marine Environmental Research, 34 (1-4), pp. 237-241. http://www.sciencedirect.com/science/article/pii/0141113692901142; https://doi.org/10.1016/0141-1136%2892%2990114-2
Abstract Glutathione S-transferase (GST) activity was present in all tissues examined of a marine flatfish, the plaice (Pleuronectesplatessa). It was predominately expressed in liver, with lower but significant activities present in the kidney, intestine and gills. Immunoblot analysis with specific antisera to GST-A and B, the two major hepatic isoforms, showed that both were expressed in all tissues except spleen; however, clear differences in the relative proportions of total GST activity and these isoforms were observed. The expression of GST-A mRNA was also investigated by a cRNA probe and followed the enzyme protein levels. The results indicate that GST-A is the predominant isoform in gills and that other, so far uncharacterised isoforms, must account for the high enzyme activity measured by conjugation of chlorodinitrobenzene in liver.