Fish metallothioneins: Molecular cloning studies and induction in cultured cells

Citation

George S, Leaver M, Frerichs GN & Burgess D (1989) Fish metallothioneins: Molecular cloning studies and induction in cultured cells. Marine Environmental Research, 28 (1-4), pp. 173-177. http://www.sciencedirect.com/science/article/pii/0141113689902195; https://doi.org/10.1016/0141-1136%2889%2990219-5

Abstract
A cDNA for plaice metallothionein (MT) was cloned and the sequence for the first 45 amino acid residues was deduced. The sequence of plaice MT was exactly homologous to that of winter flounder MT. Studies of MT induction by Cd and Zn in a rainbow trout (RTH) cell line showed that both metals were strong inducers of the protein in contrast to previous reports which have shown little induction of MT mRNA by Cd. Translational control or differential mRNA and MT stabilities are therefore inferred.

Journal
Marine Environmental Research: Volume 28, Issue 1-4