Characterization of microsatellite loci in silver carp (Hypophthalmichthys molitrix), and cross-amplification in other cyprinid species

Citation

Gheyas AA, Cairney M, Gilmour AE, Sattar MA, Das TK, McAndrew B, Penman D & Taggart J (2006) Characterization of microsatellite loci in silver carp (Hypophthalmichthys molitrix), and cross-amplification in other cyprinid species. Molecular Ecology Notes, 6 (3), pp. 656-659. https://doi.org/10.1111/j.1471-8286.2006.01288.x

Abstract
Captive populations of silver carp (Hypophthalmichthys molitrix), a major aquaculture species in Asia, would undoubtedly benefit from genetic monitoring and improvement programs. We report the isolation and preliminary characterization of 16 microsatellite loci derived from both conventional and microsatellite-enriched libraries. Inheritance studies confirmed the allelic nature of observed polymorphisms at all loci, while identifying null alleles at two loci. These loci, having varying degrees of polymorphism, should provide useful markers for applied genetic studies. A high degree of cross-amplification among 10 other cyprinid species suggests that these loci may have more widespread utility.

Keywords
cross-species amplification; genetic markers; inheritance; microsatellite enrichment; silver carp

Journal
Molecular Ecology Notes: Volume 6, Issue 3

People

Dr Almas Gheyas

Lecturer in Aquaculture Production Scien, Institute of Aquaculture