Single locus inheritance and joint segregation analysis of minisatellite (VNTR) DNA loci in brown trout (Salmo trutta L.)

Citation

Prodohl PA, Taggart J & Ferguson A (1994) Single locus inheritance and joint segregation analysis of minisatellite (VNTR) DNA loci in brown trout (Salmo trutta L.). Heredity, 73 (5), pp. 556-566. https://doi.org/10.1038/hdy.1994.155

Abstract
Eleven minisatellite (VNTR) DNA loci were examined for Mendelian inheritance and possible linkage relationships in a brown trout family (54 offspring). Analyses of the full progeny set (males and females combined) showed no statistically significant departures from Mendelian expectations at any locus, and no new mutations were observed. However, at one locus, Str-A9, differential segregation (P < 0.001) of parental alleles into male and female progeny was recorded. This is indicative of tight linkage between Str-A9 and the sex determining region in the brown trout, and confirms the male as the heterogametic sex in this species. Joint segregation analyses of 83 different pairwise comparisons of loci revealed nonrandom assortment in only two instances, i.e. both male and female comparisons of Str-A22/1 with Str-A22/2. These findings illustrate the importance of inheritance studies in clarifying the genetic basis underlying minisatellite DNA polymorphisms prior to their use as individual, familial or population markers.

Keywords
brown trout; inheritance; linkage; minisatellite DNA; Salmo trutta; VNTR

Journal
Heredity: Volume 73, Issue 5