High-efficiency detection of severe acute respiratory syndrome virus genetic material

Citation

Weidmann M, Zanotto PMDA, Weber F, Spiegel M, Brodt HR & Hufert FT (2004) High-efficiency detection of severe acute respiratory syndrome virus genetic material. Journal of Clinical Microbiology, 42 (6), pp. 2771-2773. https://doi.org/10.1128/JCM.42.6.2771-2773.2004

Abstract
A Taqman amplicon targeting the nucleocapsid gene of severe acute respiratory syndrome coronavirus (SARS-CoV) is 5 log10 times more sensitive for SARS-CoV target RNA extracted from infected cells and 2.79 log10 times more sensitive for RNA extracted from patient material of the index case in Frankfurt than an amplicon targeting the polymerase gene.

Journal
Journal of Clinical Microbiology: Volume 42, Issue 6