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Article

Recombinase Polymerase Amplification Assay for Rapid Detection of Francisella tularensis

Citation
Euler M, Wang Y, Otto P, Tomaso H, Escudero R, Anda P, Hufert FT & Weidmann M (2012) Recombinase Polymerase Amplification Assay for Rapid Detection of Francisella tularensis. Journal of Clinical Microbiology, 50 (7), pp. 2234-2238. https://doi.org/10.1128/JCM.06504-11

Abstract
Several real-time PCR approaches to develop field detection for Francisella tularensis, the infectious agent causing tularemia, have been explored. We report the development of a novel qualitative real-time isothermal recombinase polymerase amplification (RPA) assay for use on a small ESEQuant Tube Scanner device. The analytical sensitivity and specificity were tested using a plasmid standard and DNA extracts from infected rabbit tissues. The assay showed a performance comparable to real-time PCR but reduced the assay time to 10 min. The rapid RPA method has great application potential for field use or point-of-care diagnostics.

Journal
Journal of Clinical Microbiology: Volume 50, Issue 7

StatusPublished
Author(s)Euler, Milena; Wang, Yongjie; Otto, Peter; Tomaso, Herbert; Escudero, Raquel; Anda, Pedro; Hufert, Frank T; Weidmann, Manfred
Publication date31/07/2012
URLhttp://hdl.handle.net/1893/18311
PublisherAmerican Society for Microbiology
ISSN0095-1137
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