Viral load among patients infected with Marburgvirus in Angola

Citation

Weidmann M, Hufert FT & Sall AA (2007) Viral load among patients infected with Marburgvirus in Angola. Journal of Clinical Virology, 39 (1), pp. 65-66. https://doi.org/10.1016/j.jcv.2006.12.023

Abstract
First paragraph: Marburg virus (MARV) causes severe hemorrhagic fever in humans. The as yet largest outbreak occurred in Angola in 2005, with a 88% mortality rate among the 374 cases identified (Towner et al., 2006). During that outbreak, the use of real time RT-PCR in the field appeared to be critical for case management and epidemiological investigation and complementary to serological diagnostic tools (Grolla et al., 2005). In that perspective, we here report a successful update of a quantitative Taqman RT-PCR assay for MARV targeting the N gene (Weidmann et al., 2004). Using sequences newly available in GeneBank (accession numbers: DQ447649-60) the method was redesigned (MNFP 5′-ACTGARATYACACACAGTCAGACA-3′, MNRP 5′-TRTTGTTTTCAATTTCTGCAGC-3′, MNP 5′-FAM-CCGTCCTCAGCCAGAAACGAGA-TAMRA-3′) and adapted to detect the variant sequences from the Angola MARV isolates.

Keywords
Marburg virus; viral load; Angola

Notes
Output Type: Letter

Journal
Journal of Clinical Virology: Volume 39, Issue 1