Relating microfeatures of soil organic matter to C stabilisation: optical microscopy, SEM-EDS, abiotic oxidation


Falsone G, Wilson C, Cloy JM, Graham MC & Bonifacio E (2014) Relating microfeatures of soil organic matter to C stabilisation: optical microscopy, SEM-EDS, abiotic oxidation. Biology and Fertility of Soils, 50 (4), pp. 623-632.

We investigated the relationships between microscale distribution of soil organic matter (SOM) features and their stability by combining optical microscopy, SEM-EDS analysis and NaClO oxidation of soil thin sections on five soils from Harwood Forest in Northumberland (UK) differently affected by water stagnation. Plant organs at different stages of decomposition and amorphous organic matter were observed by optical microscopy in all samples. SOM microfeature distribution, size of SOM features and the relation with the C-to-N ratio suggested that amorphous features could be the end-products of organ transformation. SEM-EDS elemental analysis showed that amorphous material had higher Si/C, Al/C and Fe/C molar ratios than organs, clearly pointing to interactions with the soil inorganic phases, which contributed to SOM stabilisation. Soil porosity coupled with water stagnation seemed to affect the Fe-SOM interactions as a greater proportion of small water retention pores (10- 50 μm) was associated with higher abundance of Fe-rich amorphous organic features. The higher chemical stability of amorphous features was confirmed by oxidation. After NaClO treatment, organs were almost totally removed, while amorphous organic material was less affected both morphologically and chemically. Our results demonstrate that in water-affected soils local environment defined by the pore system affects the distribution of SOM microfeatures and that the highest resistance to oxidation of the amorphous features is attributable to the formation of organic-inorganic associations. The proposed combined approach seems to be a promising mean to investigate SOM dynamics by relating features to stability.

Organo-mineral interactions; Stagnosols; Gleysols; Soil thin sections; Soil porosity

Biology and Fertility of Soils: Volume 50, Issue 4

FundersNatural Environment Research Council
Publication date31/05/2014
Publication date online01/11/2013
Date accepted by journal21/10/2013