Development of DNA Microsatellite Loci in Chinese Carps and Application to Detection of Hybridization in Broodstock

Citation

Mia MY, Taggart J, Gilmour AE, Das TK, Sattar MA, Hussain MG, Mazid MA, McAndrew B & Penman D (2002) Development of DNA Microsatellite Loci in Chinese Carps and Application to Detection of Hybridization in Broodstock. In: Penman D, Hussain M, McAndrew B & Mazid M (eds.) Proceedings of a workshop on Genetic Management and Improvement Strategies for Exotic Carps in Asia, 12-14 February 2002, Dhaka, Bangladesh. Workshop on Genetic Management and Improvement Strategies for Exotic Carps in Asia, Dhaka, Bangladesh, 12.02.2002-14.02.2002. Mymensingh, Bangladesh: Bangladesh Fisheries Research Institute, pp. 51-57. http://r4d.dfid.gov.uk/pdf/outputs/rlaquaconfpaper6r7590.pdf

Abstract
Microsatellite DNA loci have been developed from silver carp (Hypophthalmichthys molitrix) DNA for use in broodstock management and research. Here we describe how three of these loci (Hmo1, Hmo3 and Hmo11) can be used to distinguish between silver carp and bighead carp (Aristichthys nobilis). These loci are being used in the analysis of samples collected from hatcheries in different regions of Bangladesh, in parallel with a survey of broodstock management practices. Analysis of samples from two of the hatchery areas revealed a fairly low proportion of hybrid genotypes among broodstock: this included fish which were identified as silver and bighead carp by the hatcheries. The genetic analysis suggested that while some of these fish might be F1 hybrids, others had more complex genotypes suggesting further generations of hybridization or introgression between the species. The study is ongoing and will attempt to identify factors associated with hybridization in hatchery broodstock. The methodology could also be applied to detect hybrids in nursery or ongrowing stocks.