Development of an ELISA to detect Pasteurella piscicida in culture and in 'spiked' fish tissue

Citation

Bakopoulos V, Volpatti D, Papapanagiotou E, Richards R, Galleotti M & Adams A (1997) Development of an ELISA to detect Pasteurella piscicida in culture and in 'spiked' fish tissue. Aquaculture, 156 (3-4), pp. 359-366. https://doi.org/10.1016/S0044-8486%2897%2900137-3

Abstract
An enzyme linked immunosorbent assay (ELISA) was developed to detect the presence of Pasteurella piscicida. The test, after the preparation of the solid phase, takes less than 4 h to complete and the same technique can be applied for both bacteria in culture and in tissue. The limit of sensitivity for bacteria in culture was 103 cells ml-1 or 102 cells well-1, if a plate reader is available or 105 cells ml-1 (104 cells well-1) visually. Similar sensitivity was achieved (103 cells well-1 with a plate reader, 104 cells well-1 visually) when the ELISA was performed on tissue. The technique is specific and it offers a rapid, early and sensitive means of diagnosing Pasteurellosis during outbreaks and screening large populations of fish for the presence of P. piscicida.

Keywords
Pasteurella piscicida; Monoclonal antibodies; Sandwich-ELISA

Journal
Aquaculture: Volume 156, Issue 3-4

People

Professor Randolph Richards

Emeritus Professor, Institute of Aquaculture