Peleteiro MC & Richards R (1988) Immunocytochemical studies on immunoglobulin-containing cells in the epidermis of rainbow trout Salmo gairdneri Richardson: influence of bath vaccination. Journal of Fish Biology, 32 (6), pp. 845-858. https://doi.org/10.1111/j.1095-8649.1988.tb05428.x
The presence of immunoglobulin-containing cells in the epidermis of rainbow trout was traced, using post-embedding immunocytochemical techniques, in resin-embedded fragments of skin. Light microscopy observations (for which the PAP method was used)revealed the presence of immunoglobulin-containing cells in the epidermis, although no accurate identification was possible. Electron microscopy observations (employing gold-labelling) showed that, in spite of the poor preservation thought unavoidable to protect the antigenicity of the immunoglobulins, the positive cells were either lymphocyte-like cells or mucous cells. Anti-Vibrio anguillarium antibody was detected in the epidermal mucous cells of rainbow trout juveniles vaccinated by immersion, using an immunoenzymatic sandwich technique, modified for resin-embedded tissue. The possibility of the existence of a local immune system is discussed, considering the involvement of the mucous cells.
Journal of Fish Biology: Volume 32, Issue 6