Rangdale RE, Richards R & Alderman DJ (1997) Colonisation of eyed rainbow trout ova with Flavobacterium psychrophilum leads to rainbow trout fry syndrome in fry. Bulletin of the European Association of Fish Pathologists, 17 (3-4), pp. 108-111. http://eafp.squarespace.com/bulletin-archive/1997-volume-17/issue-3-4/
Disinfected eyed rainbow trout ova from a known disease free source were incubated with Flavobacterium psychrophilium at a concentration of 1010 cells ml-1 in PBS for 30min or 60min at 10 degrees C. Eggs monitored daily for mortality until hatching began. The subsequent fry were reared separately during which time swim-up and post swim-up mortality were recorded. No statistical differences were detected in either egg mortality, or swim-up mortality between artificially colonised and control groups. However, fry which orginated from artificially colonised eggs showed significantly higher mortality, clinical signs of rainbow trout fry syndrome and concommittally F. psychrophilum was detected from viscera.
Bulletin of the European Association of Fish Pathologists: Volume 17, Issue 3-4