Detection of SJNNV and RGNNV genotypes using a relative quantification RT-PCR assay

Citation

Cherif N, Lopez Jimena B, Garcia-Rosado E, Cano I, Castro D, Borrego JJ, Alonso MdC & Hammami S (2011) Detection of SJNNV and RGNNV genotypes using a relative quantification RT-PCR assay. Journal of Applied Ichthyology, 27 (3), pp. 805-812. https://doi.org/10.1111/j.1439-0426.2010.01592.x

Abstract
Viral Encephalopathy and Retinopathy (VER), is caused by a nodavirus included within the Betanodavirus genus of the Nodaviridae family. This disease affects more than 30 marine fish species worldwide and has been a major obstacle in the aquaculture industry; control of the disease is based on virus detection, essentially in carrier specimens. This study describes a real time PCR procedure for viral nervous necrosis virus detection from several organs of sea bass, Senegalese sole, and gilt-head sea bream, from fish displaying either clinical symptoms or asymptomatic cases. The sensitivity of this technique was about 106-fold higher than that of the conventional RT-PCR. The newly designed primers detected nodavirus isolates belonging to the RGNNV and SJNNV genotypes.

Journal
Journal of Applied Ichthyology: Volume 27, Issue 3