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Article

Rapid detection of shrimp white spot syndrome virus by real time, isothermal recombinase polymerase amplification assay

Citation
Xia X, Yu Y, Weidmann M, Pan Y, Yan S & Wang Y (2014) Rapid detection of shrimp white spot syndrome virus by real time, isothermal recombinase polymerase amplification assay. PLoS ONE, 9 (8), Art. No.: e104667. https://doi.org/10.1371/journal.pone.0104667

Abstract
White spot syndrome virus (WSSV) causes large economic losses to the shrimp aquaculture industry, and thus far there are no efficient therapeutic treatments available against this lethal virus. In this study, we present the development of a novel real time isothermal recombinase polymerase amplification (RPA) assay for WSSV detection on a small ESEQuant Tube Scanner device. The RPA sensitivity, specificity and rapidity were evaluated by using a plasmid standard as well as viral and shrimp genomic DNAs. Compared with qPCR, the RPA assay revealed more satisfactory performance. It reached a detection limit up to 10 molecules in 95% of cases as determined by probit analysis of 8 independent experiments within 6.41±0.17 min at 39°C. Consequently, this rapid RPA method has great application potential for field use or point of care diagnostics.

Journal
PLoS ONE: Volume 9, Issue 8

StatusPublished
Author(s)Xia, Xiaoming; Yu, Yongxin; Weidmann, Manfred; Pan, Yingjie; Yan, Shuling; Wang, Yongjie
Publication date31/12/2014
Date accepted by journal11/07/2014
URLhttp://hdl.handle.net/1893/21078
PublisherPublic Library of Science
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