An intron is required for dihydrofolate reductase protein stability



Noe V, MacKenzie S & Ciudad CJ (2003) An intron is required for dihydrofolate reductase protein stability. Journal of Biological Chemistry, 278 (40), pp. 38292-38300.

We compared the expression of dihydrofolate reductase minigenes with and without an intron. The levels of protein were significantly higher in the presence of dihydrofolate reductase intron 1. However, mRNA levels in both constructs were comparable. In addition, the RNA transcribed from either construct was correctly polyadenylated and exported to the cytoplasm. The intron-mediated increase in dihydrofolate reductase protein levels was position-independent and was also observed when dihydrofolate reductase intron 1 was replaced by heterologous introns. The translational rate of dihydrofolate reductase protein was increased in transfectants from the intron-containing minigene. In addition, the protein encoded by the intronless construct was unstable and subject to lysosomal degradation, thus showing a shorter half-life than the protein encoded by the intron-containing minigene. We conclude that an intron is required for the translation and stability of dihydrofolate reductase protein.

Journal of Biological Chemistry: Volume 278, Issue 40

Publication date31/10/2003
PublisherAmerican Society for Biochemistry and Molecular Biology

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Professor Simon MacKenzie

Professor Simon MacKenzie

Professor & Head of Inst of Aquaculture, Institute of Aquaculture