Article

Shotgun proteomics: concept, key points and data mining

Citation

Matallana-Surget S, Baptiste L & Wattiez R (2010) Shotgun proteomics: concept, key points and data mining. Expert Review of Proteomics, 7 (1), pp. 5-7. https://doi.org/10.1586/epr.09.101

Abstract
First paragraph: Mass spectrometry (MS)-based approaches are used increasingly to address diverse questions in proteomics research, enabling one to comprehensively analyze complex samples of proteins on a global level. Owing to all the effort devoted to explore entire genomes, we now have access to a multitude of completed genome sequences, which has recently opened up a new era of ‘shotgun proteome sequencing’. The concept of shotgun proteomics basically consists of the multidimensional separation (MudPIT) of a complex mixture of peptides, generated from the treatment of a specific protease (e.g., trypsin), analyzed using MS/MS and then submitted to automated database searching.

Notes
Output Type: Editorial

Journal
Expert Review of Proteomics: Volume 7, Issue 1

StatusPublished
Publication date31/12/2010
Publication date online09/01/2014
Date accepted by journal09/01/2014
URLhttp://hdl.handle.net/1893/24725
PublisherTaylor and Francis
ISSN1478-9450