Article

Identification and validation of single nucleotide polymorphisms as tools to detect hybridization and population structure in freshwater stingrays

Citation

Cruz VP, Vera M, Pardo BG, Taggart J, Martinez P, Oliveira C & Foresti F (2017) Identification and validation of single nucleotide polymorphisms as tools to detect hybridization and population structure in freshwater stingrays. Molecular Ecology Resources, 17 (3), pp. 550-556. https://doi.org/10.1111/1755-0998.12564

Abstract
Single nucleotide polymorphism (SNP) markers were identified and validated for two stingrays species, Potamotrygon motoro and Potamotrygon falkneri, using double digest restriction-site associated DNA (ddRAD) reads using 454-Roche technology. A total of 226774 reads (65.5Mb) were obtained (mean read length 289±183bp) detecting a total of 5399 contigs (mean contig length: 396±91bp). Mining this data set, a panel of 143 in silico SNPs was selected. Eighty-two of these SNPs were successfully validated and 61 were polymorphic: 14 in P.falkneri, 21 in P.motoro, 3 in both species and 26 fixed for alternative variants in both species, thus being useful for population analyses and hybrid detection.

Keywords
conservation tools; genetic fish; hybridization; NGS techniques; Potamotrygon ; SNPs

Journal
Molecular Ecology Resources: Volume 17, Issue 3

StatusPublished
Publication date31/05/2017
Publication date online28/07/2016
Date accepted by journal07/01/2016
URLhttp://hdl.handle.net/1893/25544
PublisherWiley-Blackwell
ISSN1755-098X