Article

Using systems biology to define the essential biological networks responsible for adaptation to endurance exercise training

Citation

Keller P, Vollaard N, Babraj J, Ball D, Sewell DA & Timmons JA (2007) Using systems biology to define the essential biological networks responsible for adaptation to endurance exercise training. Biochemical Society Transactions, 35 (5), pp. 1306-1309. https://doi.org/10.1042/BST0351306

Abstract
We predict that RNA level regulation is as diverse and powerful as protein level regulation when considering physiological adaptation. Non-coding RNA molecules, such as miRNAs (microRNAs), have emerged as a powerful mechanism for post-transcriptional regulation of mRNA. In an effort to define the role of miRNA in human skeletal-muscle biology, we have initiated profiling of muscle RNA before and after endurance exercise training. The robust molecular phenotype of muscle is established using unbiased analysis strategies of the raw data, reflecting the statistical power of gene ontology and network analysis. We can thus determine the structural features of the skeletal-muscle transcriptome, identify discrete networks activated by training and utilize bioinformatics predictions to establish the interaction between non-coding RNA modulation and Affymetrix expression profiles.

Keywords
affymetrix; endurance exercise; integrin; microRNA; non-coding RNA; systems biology

Journal
Biochemical Society Transactions: Volume 35, Issue 5

StatusPublished
Publication date01/11/2007
PublisherPortland Press
ISSN0300-5127