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Article

Control of crystal polymorph in microfluidics using molluscan 28 kDa Ca2+-binding protein

Citation
Ji B, Cusack M, Freer A, Gadegaard N & Yin H (2010) Control of crystal polymorph in microfluidics using molluscan 28 kDa Ca2+-binding protein. Integrative Biology, 2 (10), pp. 528-535. https://doi.org/10.1039/c0ib00007h

Abstract
Biominerals produced by biological systems in physiologically relevant environments possess extraordinary properties that are often difficult to replicate under laboratory conditions. Understanding the mechanism that underlies the process of biomineralisation can lead to novel strategies in the development of advanced materials. Using microfluidics, we have demonstrated for the first time, that an extrapallial (EP) 28 kDa protein, located in the extrapallial compartment between mantle and shell of Mytilus edulis, can influence, at both micro- and nanoscopic levels, the morphology, structure and polymorph that is laid down in the shell ultrastructure. Crucially, this influence is predominantly dependent on the existence of an EP protein concentration gradient and its consecutive interaction with Ca2+ ions. Novel lemon-shaped hollow vaterite structures with a clearly defined nanogranular assembly occur only where particular EP protein and Ca2+ gradients co-exist. Computational fluid dynamics enabled the progress of the reaction to be mapped and the influence of concentration gradients across the device to be calculated. Importantly, these findings could not have been observed using conventional bulk mixing methods. Our findings not only provide direct experimental evidence of the potential influence of EP proteins in crystal formation, but also offer a new biomimetic strategy to develop functional biomaterials for applications such as encapsulation and drug delivery. © 2010 The Royal Society of Chemistry.

Journal
Integrative Biology: Volume 2, Issue 10

StatusPublished
Author(s)Ji, Bozhi; Cusack, Maggie; Freer, Andy; Gadegaard, Nikolaj; Yin, Huabing
Publication date31/10/2010
Publication date online27/08/2010
Date accepted by journal23/07/2010
URLhttp://hdl.handle.net/1893/25074
PublisherRoyal Society of Chemistry
ISSN1093-4391
eISSN1757-9708
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