Validation of a KHV antibody enzyme-linked immunosorbent assay (ELISA)



Bergmann SM, Wang Q, Zeng W, Li Y, Wang Y, Matras M, Reichert M, Fichtner D, Lenk M, Morin T, Olesen NJ, Skall HF, Lee P, Zheng S & Monaghan S (2017) Validation of a KHV antibody enzyme-linked immunosorbent assay (ELISA). Journal of Fish Diseases, 40 (11), pp. 1511-1527.

Koi herpesvirus (KHV) causes KHV disease (KHVD). The virus is highly contagious in carp or koi and can induce a high mortality. Latency and, in some cases, a lack of signs presents a challenge for virus detection. Appropriate immunological detection methods for anti-KHV antibodies have not yet been fully validated for KHV. Therefore, it was developed and validated an enzyme-linked immunosorbent assay (ELISA) to detect KHV antibodies. The assay was optimized with respect to plates, buffers, antigens and assay conditions. It demonstrated high diagnostic and analytical sensitivity and specificity and was particularly useful at the pond or farm levels. Considering the scale of the carp and koi industry worldwide, this assay represents an important practical tool for the indirect detection of KHV, also in the absence of clinical signs.

carp and koi sera; enzyme-linked immunosorbent assay; koi herpesvirus; koi herpesvirus antibodies

Additional co-authors: S Reiche, W Fuchs, M Kotler, K Way, G Bräuer, K Böttcher, A Kappe, and J Kielpinska

Journal of Fish Diseases: Volume 40, Issue 11

Publication date30/11/2017
Publication date online07/05/2017
Date accepted by journal20/01/2017

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Dr Sean Monaghan

Dr Sean Monaghan

Senior Lecturer, Institute of Aquaculture