A versatile nuclei extraction protocol for single nucleus sequencing in non-model species–Optimization in various Atlantic salmon tissues

Citation

Ruiz Daniels R, Taylor RS, Dobie R, Salisbury S, Furniss JJ, Clark E, Macqueen DJ & Robledo D (2023) A versatile nuclei extraction protocol for single nucleus sequencing in non-model species–Optimization in various Atlantic salmon tissues. Winter S (Editor) PLOS ONE, 18 (9), Art. No.: e0285020. https://doi.org/10.1371/journal.pone.0285020

Abstract
The use of single cell sequencing technologies has exploded over recent years, and is now commonly used in many non-model species. Sequencing nuclei instead of whole cells has become increasingly popular, as it does not require the processing of samples immediately after collection. Here we present a highly effective nucleus isolation protocol that outperforms previously available method in challenging samples in a non-model specie. This protocol can be successfully applied to extract nuclei from a variety of tissues and species.

Keywords
Multidisciplinary

Journal
PLOS ONE: Volume 18, Issue 9

People

Dr Rose Ruiz Daniels

Lecturer in Aquaculture Genomics, Institute of Aquaculture