Article

Pharaoh Cuttlefish, Sepia pharaonis, Genome Reveals Unique Reflectin Camouflage Gene Set

Citation

Song W, Li R, Zhao Y, Migaud H, Wang C & Bekaert M (2021) Pharaoh Cuttlefish, Sepia pharaonis, Genome Reveals Unique Reflectin Camouflage Gene Set. Frontiers in Marine Science, 8, Art. No.: 639670. https://doi.org/10.3389/fmars.2021.639670

Abstract
Sepia pharaonis, the pharaoh cuttlefish, is a commercially valuable cuttlefish species across the southeast coast of China and an important marine resource for the world fisheries. Research efforts to develop linkage mapping, or marker-assisted selection have been hampered by the absence of a high-quality reference genome. To address this need, we produced a hybrid reference genome of S. pharaonis using a long-read platform (Oxford Nanopore Technologies PromethION) to assemble the genome and short-read, high quality technology (Illumina HiSeq X Ten) to correct for sequencing errors. The genome was assembled into 5,642 scaffolds with a total length of 4.79 Gb and a scaffold N50 of 1.93 Mb. Annotation of the S. pharaonis genome assembly identified a total of 51,541 genes, including 12 copies of the reflectin gene, that enable cuttlefish to control their body coloration. This new reference genome for S. pharaonis provides an essential resource for future studies into the biology, domestication and selective breeding of the species.

Keywords
Sepia pharaonis; cephalopod; sequencing; genome; mitochondria; reflectin

Journal
Frontiers in Marine Science: Volume 8

StatusPublished
FundersBBSRC Biotechnology and Biological Sciences Research Council
Publication date31/12/2021
Publication date online15/02/2021
Date accepted by journal26/01/2021
URLhttp://hdl.handle.net/1893/32292
PublisherFrontiers Media SA
eISSN10.3389/fmars.2021.639670