Comparative Analysis of Zika Virus Detection by RT-qPCR, RT-LAMP, and RT-RPA

Citation

Diagne CT, Faye M, Lopez-Jimena B, Abd El Wahed A, Loucoubar C, Fall C, Mencatelli G, Faye O, Faye O, Weidmann M & Sall AA (2020) Comparative Analysis of Zika Virus Detection by RT-qPCR, RT-LAMP, and RT-RPA. In: Kobinger G & Racine T (eds.) Zika Virus: Methods and Protocols. Methods in Molecular Biology, 2142. New York: Springer, pp. 165-179. https://doi.org/10.1007/978-1-0716-0581-3_14

Abstract
Molecular detection of Zika virus (ZIKV) is a key element of outbreak management. Multiple PCR and isothermal ZIKV assays targeting different ZIKV sequences have been published. In this study, we compared a qRT-PCR, 2 RT-LAMP assays (based on different primer design approaches), and an RT-RPA for the detection of African and Asian/American lineages of ZIKV isolates from human, mosquito, and monkey. Results showed that RT-LAMP detected 100% of samples with a time threshold (Tt) of 18.01 ± 11.71 min while qRT-PCR detected 88.88% of samples with a Tt of 58.30 ± 16.58 min and RT-RPA 50% of samples with a Tt of 3.70 ± 0.44 min.

Keywords
Diagnostics; Zika virus; Isothermal amplification; RT-LAMP; RT-PCR; RT-RPA