Werner J, Géron A, Kerssemakers J & Matallana-Surget S (2019) mPies: a novel metaproteomics tool for the creation of relevant protein databases and automatized protein annotation. Biology Direct, 14 (1), Art. No.: 21. https://doi.org/10.1186/s13062-019-0253-x
Metaproteomics allows to decipher the structure and functionality of microbial communities. Despite its rapid development, crucial steps such as the creation of standardized protein search databases and reliable protein annotation remain challenging. To overcome those critical steps, we developed a new program named mPies (metaProteomics in environmental sciences). mPies allows the creation of protein databases derived from assembled or unassembled metagenomes, and/or public repositories based on taxon IDs, gene or protein names. For the first time, mPies facilitates the automatization of reliable taxonomic and functional consensus annotations at the protein group level, minimizing the well-known protein inference issue, which is commonly encountered in metaproteomics. mPies’ workflow is highly customizable with regards to input data, workflow steps, and parameter adjustment. mPies is implemented in Python 3/Snakemake and freely available on GitHub: https://github.com/johanneswerner/mPies/.
Bioinformatics; Metaproteomics; Microbial ecology; Protein annotation; Protein search database
Biology Direct: Volume 14, Issue 1
|Publication date online||14/11/2019|
|Date accepted by journal||02/10/2019|
|Publisher||Springer Science and Business Media LLC|