Molecular Diagnostics of Ebola Patient Samples by Institut Pasteur de Dakar Mobile Laboratory in Guinea 2014-2016

Citation

Faye O, Diagne CT, Diallo A, Meyer E, Soropogui B, Fall G, Fall C, Magassouba N, Koivogui L, Keita S, Loucoubar C, Diop M, Weidmann M, Faye O & Sall AA (2020) Molecular Diagnostics of Ebola Patient Samples by Institut Pasteur de Dakar Mobile Laboratory in Guinea 2014-2016. In: Okware SI (ed.) Re-emerging Filovirus Diseases. London: InTech, pp. 71-81. https://doi.org/10.5772/intechopen.88263

Abstract
As part of the laboratory response to the Ebola virus outbreak in Guinea, the Institut Pasteur de Dakar mobile laboratory (IPD-ML) was set up in Donka hospital from 2014 to 2016. EBOV suspected samples collected at Ebola Treatment Centers (ETC) and from community deaths were sent daily to IPD-ML. Analysis was performed using dried oligonucleotide mixes for real-time RT-PCR designed for field diagnostic. From March 2014 to May 2015, a total of 6055 patient samples suspected for EBOV collected from seven regions of Guinea were tested by real-time RT-PCR. These patients’ clinical included serum samples (n = 2537 samples) and swabs (n = 3518 samples) with positivity rates of 36.74 and 6.88% respectively. Females were significantly more affected than males with positivity rates of 22.39 and 17.22% respectively (p-value = 5.721e-7). All age groups were exposed to the virus with significant difference (p-value

Keywords
Ebola disease virus; West-Africa epidemic; mobile laboratory deployment; social benefits