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Article

Expanding the miRNA Repertoire in Atlantic Salmon; Discovery of IsomiRs and miRNAs Highly Expressed in Different Tissues and Developmental Stages

Citation
Woldemariam NT, Agafonov O, Hoyheim B, Houston RD, Taggart JB & Andreassen R (2019) Expanding the miRNA Repertoire in Atlantic Salmon; Discovery of IsomiRs and miRNAs Highly Expressed in Different Tissues and Developmental Stages. Cells, 8 (1), Art. No.: 42. https://doi.org/10.3390/cells8010042

Abstract
MicroRNAs (miRNAs) are important post-transcriptional gene expression regulators. Here, 448 different miRNA genes, including 17 novel miRNAs, encoding for 589 mature Atlantic salmon miRNAs were identified after sequencing 111 samples (fry, pathogen challenged fry, various developmental and adult tissues). This increased the reference miRNAome with almost one hundred genes. Prior to isomiR characterization (mature miRNA variants), the proportion of erroneous sequence variants (ESVs) arising in the analysis pipeline was assessed. The ESVs were biased towards 5’ and 3’ end of reads in unexpectedly high proportions indicating that measurements of ESVs rather than Phred score should be used to avoid misinterpreting ESVs as isomiRs. Forty-three isomiRs were subsequently discovered. The biological effect of the isomiRs measured as increases in target diversity was small (

Keywords
Teleostei; embryogenesis; tissue-enriched miRNAs; post-transcriptional gene regulation;

Journal
Cells: Volume 8, Issue 1

StatusPublished
Author(s)Woldemariam, Nardos Tesfaye; Agafonov, Oleg; Hoyheim, Bjørn; Houston, Ross D; Taggart, John B; Andreassen, Rune
FundersNorwegian Research Council
Publication date11/01/2019
Publication date online11/01/2019
Date accepted by journal18/12/2018
URLhttp://hdl.handle.net/1893/28654
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