Citation Hussain MG, Penman D, McAndrew B & Johnstone R (1993) Suppression of first cleavage in the Nile tilapia, Oreochromis niloticus L. -- a comparison of the relative effectiveness of pressure and heat shocks, Aquaculture, 111 (1-4), pp. 263-270.
Abstract Evidence is presented for the successful suppression of first cleavage in Oreochromis niloticus L. and thereby the production of mitotic gynogens. The optimal parameters for UV irradiation of milt were 300-310 μW cm-2 for 2 min at 4°C. The optimal pressure shock parameters were 630 kg cm-2 (9000 psi) for 2 min at 28°C at 40-50 min after fertilization (AF) and those for heat shock were 41°C for 3.5 min at 27.5-30 min AF. Isozyme analysis of survivors at two loci, ADA∗ and EST-2∗, confirmed homozygosity and lack of paternal inheritance.
Hussain M Gulam, Penman David, McAndrew Brendan, Johnstone Ray