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Regulation of hepatic glutathione S-transferase expression in flounder

Scott K, George S & Leaver M (1992) Regulation of hepatic glutathione S-transferase expression in flounder, Marine Environmental Research, 34 (1-4), pp. 233-236.

The major glutathione S-transferase isoform of flounder liver, an antigenically related structural homologue of plaice GST-A, also displays mRNA homology. A 901bp cRNA probe for plaice GST-A cross-hybridised to a 1100bp flounder mRNA on northern blot analysis. The plaice antibody and cRNA probes were used to study effects of inducer treatment on GST-A expression in flounder liver. Six days after PAH treatment (3-methylcholanthrene) total hepatic GST activity was halved, levels of GST-A were 80% and GST-A mRNA levels were 25% of controls. A commercial PCB mixture (Aroclor 1254TM) had little effect on total GST or GST-A levels despite halving GST-A mRNA levels. An epoxide, trans-stilbene oxide induced total GST activity 1·4 fold and GST-A protein levels 1·8-fold and its mRNA levels 3-fold. This reduced expression of the major flounder hepatic GST by agents which induce cytochrome P4501A1 may modulate cytoxicity of environmental pollutants in this species.

AuthorsScott Karen, George Stephen, Leaver Michael
Publication date1992
Publication date online15/04/2003
ISSN 0141-1136

Marine Environmental Research: Volume 34, Issue 1-4 (1992)

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