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Biosynthesis of very long-chain fatty acids (C > 24) in Atlantic salmon: Cloning, functional characterisation, and tissue distribution of an Elovl4 elongase

Citation
Carmona-Antonanzas G, Monroig O, Dick JR, Davie A & Tocher DR (2011) Biosynthesis of very long-chain fatty acids (C > 24) in Atlantic salmon: Cloning, functional characterisation, and tissue distribution of an Elovl4 elongase, Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 159 (2), pp. 122-129.

Abstract
The elongases of very long-chain fatty acids (Elovl) account for the rate-limiting condensation step of the elongation process in fatty acid (FA) biosynthesis in vertebrates. One member of the Elovl family, Elovl4, has been regarded as a critical enzyme in vertebrates in the production of the so-called very long-chain fatty acids (VLC-FA), a group of compounds that have been scarcely explored in fish. Here we report on the cloning of a novel elovl4-like elongase from Atlantic salmon. The salmon elovl4 cDNA codes for a putative protein containing 306 amino acids. Heterologous expression in yeast demonstrated that salmon Elovl4 efficiently elongated saturated FAs up to 36:0, with 24:0 and 26:0 appearing as preferred substrates. Additionally, salmon Elovl4 effectively converted C20 and C22 polyunsaturated fatty acids to elongated polyenoic products up to C36. Tissue distribution showed that elovl4 mRNA transcripts are abundant in eye, brain and testes, suggesting that, as described in mammals, these tissues are important metabolic sites for the biosynthesis of VLC-FA. Our results are discussed in comparison with the functional analyses observed in Elovl4 proteins from other vertebrates, and also other Elovl proteins investigated previously in Atlantic salmon.

Keywords
Atlantic salmon; fatty acid metabolism; Very long chain polyunsaturated fatty acid; biosynthesis; Elovl4 elongase; cDNA cloning; Functional characterisation; Yeast

Subject headings
Atlantic salmon; Fishes Feeding and feeds; Fishes Nutrition.; Lipids in nutrition

StatusPublished
AuthorsCarmona-Antonanzas Greta, Monroig Oscar, Dick James R, Davie Andrew, Tocher Douglas R
Publication date06/2011
Date accepted by journal28/02/2011
URLhttp://www.sciencedirect.com/science/journal/10964959
PublisherElsevier
ISSN 1096-4959
LanguageEnglish

Journal
Comparative Biochemistry and Physiology - Part b: Biochemistry and Molecular Biology: Volume 159, Issue 2 (2011-06)

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