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Proteome-wide analysis and diel proteomic profiling in the cyanobacterium Arthrospira platensis PCC 8005

Matallana-Surget S, Derock J, Baptiste L, Badri H, Deschoenmaeker F & Wattiez R (2014) Proteome-wide analysis and diel proteomic profiling in the cyanobacterium Arthrospira platensis PCC 8005, PLoS ONE, 9 (6), Art. No.: e99076.

The filamentous cyanobacteriumArthrospira platensishas a long history of use as a food supply and it has been used by the European Space Agency in the MELiSSA project, an artificial microecosystem which supports life during long-term manned space missions. This study assesses progress in the field of cyanobacterial shotgun proteomics and light/dark diurnal cycles by focusing onArthrospira platensis. Several fractionation workflows including gel-free and gel-based protein/peptide fractionation procedures were used and combined with LC-MS/MS analysis, enabling the overall identification of 1306 proteins, which represents 21% coverage of the theoretical proteome. A total of 30 proteins were found to be significantly differentially regulated under light/dark growth transition. Interestingly, most of the proteins showing differential abundance were related to photosynthesis, the Calvin cycle and translation processes. A novel aspect and major achievement of this work is the successful improvement of the cyanobacterial proteome coverage using a 3D LC-MS/MS approach, based on an immobilized metal affinity chromatography, a suitable tool that enabled us to eliminate the most abundant protein, the allophycocyanin. We also demonstrated that cell growth follows a light/dark cycle inA. platensis. This preliminary proteomic study has highlighted new characteristics of theArthrospira platensisproteome in terms of diurnal regulation.

AuthorsMatallana-Surget Sabine, Derock Jérémy, Baptiste Leroy, Badri Hanene, Deschoenmaeker Frederic, Wattiez Ruddy
Publication date10/06/2014
Publication date online10/06/2014
Date accepted by journal09/05/2014
PublisherPublic Library of Science
ISSN 1932-6203

Plos one: Volume 9, Issue 6

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