Professor
Institute of Aquaculture Pathfoot Building, University of Stirling, Stirling, FK9 4LA
http://www.stir.ac.uk/people/drtocher
Professor Douglas R. Tocher has a BSc (1979) and PhD (1982) in Biochemistry from the University of Edinburgh, Scotland. He worked for 16 years for the UK Natural Environment Research Council (NERC) at the Institute of Marine Biochemistry, Aberdeen (1982-1986) and the Unit of Aquatic Biochemistry, University of Stirling (1986-1998). His main research interests during this period were fish lipid and fatty acid metabolism including embryonic and early larval development, and cell culture studies on eicosanoid metabolism, polyunsaturated fatty acid synthesis and neural development. In 1998 he joined the Nutrition Group, Institute of Aquaculture, University of Stirling as senior lecturer and was awarded his personal chair in 2009. His current primary research interests are the molecular biology and genetic basis of regulation of lipid and fatty acid metabolism and nutrition in fish, with particular focus on omega-3 (n-3) long-chain polyunsaturated fatty acids. Professor Tocher has co-authored over 300 scientific publications.
Polyunsaturated fatty-acids in marine and terrestrial food webs. 11th Conference of the European Society for Comparative Physiology and Biochemistry, and 1st Joint Meeting ESCPB - Association Des Physiologistes
European Society for Comparative Physiology and Biochemistry
The work is directed to the research theme "Fish as Food" with the major drivers being sustainabilty of fish farming, while maintenance of health nenefits of fish, and food security. The overarching aim is to develop alternative, more sustainable feeds not dependent upon finite and limited marine resources (fishmeal and fish oil) with a paricular focus on alterantive supplies of omega-3 (n-3) long-chain polyunsaturated fatty acids. Specific aims of current research are focused on the molecular biology of lipids/fatty acids, particularly hepatic microsomal fatty acyl desaturases and polyunsaturated fatty acid elongases and transcription factors and nuclear receptors including peroxisome proliferator activated receptors (PPARs), sterol regulatory element binding proteins (SREBP) and lipid X receptors (LXR) involved in lipid/fatty acid pathway regulation. Application of post-genomic technologies to determine gene expression in various studies of fish lipid metabolism and nutrition including development and utilization of salmon oligonucleotide microarrays and next generation sequencing.
Investigating the potential of nutritional programming to improve the utilisation of sustainable feeds in aquaculture
PI: Dr Monica Betancor
Funded by: Biomar Ltd and Biotechnology and Biological Sciences Research Council
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Optimising dietary fatty acids and lipids of Atlantic salmon to secure their health and welfare thorugh varying environmental conditions and life stages (Optihealth)
PI: Professor Brett Glencross
Funded by: The Norwegian Seafood Research Fund
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ARRAINA
PI: Professor Douglas Tocher
Funded by: European Commission (Horizon 2020)
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Development of a Protein-Rich and Starch-Rich Fractions from Faba Beans for Salmon and Terrestrial Animal Production,
PI: Professor Douglas Tocher
Funded by: Technology Strategy Board
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Evaluating novel plant oilseeds enriched in omega-3 long-chain polyunsaturated fatty acids to support sustainable development of aquaculture
PI: Professor Douglas Tocher
Funded by: Biomar Ltd
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Evaluating novel plant oilseeds enriched in omega-3 long-chain polyunsaturated fatty acids to support sustainable development of aquaculture
PI: Professor Douglas Tocher
Funded by: Biomar Ltd and Biotechnology and Biological Sciences Research Council
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SAIC Equipment Award
PI: Professor Jimmy Turnbull
Funded by: Scottish Aquaculture Innovation Centre
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AQUAMAX: Sustainable Aquafeeds to maximise the health benefits of farmed fish for consumers
PI: Professor Gordon Bell
Funded by: European Commission (Horizon 2020)
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Oxidation, Lipids, DNA and Mitochondria
PI: Professor Douglas Tocher
Funded by: European Commission (Horizon 2020)
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Validation of Fourier-transform infrared spectroscopy as a novel trait analysis tool in salmon to quantify Omega-3 fatty acids
PI: Professor Douglas Tocher
Funded by: Biosciences Knowledge Transfer Network
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Lipid Fatty Acid metabolism and Nutrition in Fish
PI: Professor Douglas Tocher
Funded by: Royal Society
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New Marine Feed Resources: The Building of a consortium towards exploitation of under-utilised and un-utilised marine resources for diets to farmed fish
PI: Professor Douglas Tocher
Funded by: Institute of Marine Research
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COBIAGENE.
PI: Professor Douglas Tocher
Funded by: European Commission (Horizon 2020)
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Marie Curie IEF Fishinutrigen
PI: Professor Douglas Tocher
Funded by: European Commission (Horizon 2020)
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Study of the desaturases of omega- 3 highly unsaturated fatty acid (HUFA) biosynthesis in the Cobia (Raxhycentron Canadum)
PI:
Funded by: The Royal Society of Edinburgh
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Transcriptional control of polyunsaturated fatty acid synthesis in fish
PI: Professor Douglas Tocher
Funded by: Biotechnology and Biological Sciences Research Council
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Functional Characterisation of fatty acyl desaturase cDNA from sea bass by heterologous expression in yeast
PI: Professor Douglas Tocher
Funded by: The Carnegie Trust
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Article
Wang X, Jin M, Cheng X, Hu X, Zhao M, Yuan Y, Sun P, Jiao L, Betancor M, Tocher D & Zhou Q (2021) Dietary DHA/EPA ratio affects growth, tissue fatty acid profiles and expression of genes involved in lipid metabolism in mud crab Scylla paramamosain supplied with appropriate n-3 LC-PUFA at two lipid levels. Aquaculture, 532, Art. No.: 736028. https://doi.org/10.1016/j.aquaculture.2020.736028
Article
Shi B, Yuan Y, Jin M, Betancor MB, Tocher DR, Jiao L, Song D & Zhou Q (2021) Transcriptomic and physiological analyses of hepatopancreas reveal the key metabolic changes in response to dietary copper level in Pacific white shrimp Litopenaeus vannamei. Aquaculture, 532, Art. No.: 736060. https://doi.org/10.1016/j.aquaculture.2020.736060
Article
Betancor M, MacEwan A, Sprague M, Gong X, Montero D, Han L, Napier J, Norambuena F, Izquierdo M & Tocher D (2021) Oil from transgenic Camelina sativa as a source of EPA and DHA in feed for European sea bass (Dicentrarchus labrax L.). Aquaculture, 530, Art. No.: 735759. https://doi.org/10.1016/j.aquaculture.2020.735759
Article
Guo H, Chen C, Yan X, Li Y, Wen X, You C, Monroig Ó, Tocher DR & Wang S (2021) Effects of different dietary oil sources on growth performance, antioxidant capacity and lipid deposition of juvenile golden pompano Trachinotus ovatus. Aquaculture, 530, Art. No.: 735923. https://doi.org/10.1016/j.aquaculture.2020.735923
Article
Sprague M, Fawcett S, Betancor M, Struthers W & Tocher D (2020) Variation in the nutritional composition of farmed Atlantic salmon (Salmo salar L.) fillets with emphasis on EPA and DHA contents. Journal of Food Composition and Analysis, 94, Art. No.: 103618. https://doi.org/10.1016/j.jfca.2020.103618
Article
Chen C, Zhang M, Li Y, Wang S, Xie D, Wen X, Hu Y, Shen J, He X, You C, Tocher DR & Monroig Ó (2020) Identification of miR-145 as a Key Regulator Involved in LC-PUFA Biosynthesis by Targeting hnf4α in the Marine Teleost Siganus canaliculatus. Journal of Agricultural and Food Chemistry, 68 (51), pp. 15123-15133. https://doi.org/10.1021/acs.jafc.0c04649
Article
Saito T, Whatmore P, Taylor JF, Fernandes JMO, Adam A, Tocher DR, Espe M & Skjærven KH (2020) Micronutrient supplementation affects transcriptional and epigenetic regulation of lipid metabolism in a dose-dependent manner. Epigenetics. https://doi.org/10.1080/15592294.2020.1859867
Article
Vera LM, Hamre K, Espe M, Hemre G, Skjærven K, Lock E, Prabhu AJ, Leeming D, Migaud H, Tocher DR & Taylor JF (2020) Higher dietary micronutrients are required to maintain optimal performance of Atlantic salmon (Salmo salar) fed a high plant material diet during the full production cycle. Aquaculture, 528, Art. No.: 735551. https://doi.org/10.1016/j.aquaculture.2020.735551
Article
Brugere C, Padmakumar K, Leschen W & Tocher DR (2020) What influences the intention to adopt aquaculture innovations? Concepts and empirical assessment of fish farmers' perceptions and beliefs about aquafeed containing non-conventional ingredients. Aquaculture Economics and Management. https://doi.org/10.1080/13657305.2020.1840661
Article
Can mesopelagic mixed layers be used as feed sources for salmon aquaculture?
Olsen RE, Strand E, Melle W, Nørstebø J, Lall S, Ringø E, Tocher DR & Sprague M (2020) Can mesopelagic mixed layers be used as feed sources for salmon aquaculture?. Deep Sea Research Part II: Topical Studies in Oceanography, 180, Art. No.: 104722. https://doi.org/10.1016/j.dsr2.2019.104722
Article
Shi B, Jin M, Jiao L, Betancor MB, Tocher DR & Zhou Q (2020) Effects of dietary zinc level on growth performance, lipolysis and expression of genes involved in the calcium/calmodulin-dependent protein kinase kinase-β/AMP-activated protein kinase pathway in juvenile Pacific white shrimp. British Journal of Nutrition, 124 (8), pp. 773-784. https://doi.org/10.1017/s0007114520001725
Article
Luo J, Zhu T, Jin M, Cheng X, Yuan Y, Wang X, Lu J, Jiao L, Tocher DR & Zhou Q (2020) Influence of dietary zinc on growth, zinc bioaccumulation and expression of genes involved in antioxidant and innate immune in juvenile mud crabs (Scylla paramamosain). British Journal of Nutrition, 124 (7), pp. 681-692. https://doi.org/10.1017/s0007114520001531
Article
Chen C, Wang S, Hu Y, Zhang M, He X, You C, Wen X, Monroig O, Tocher DR & Li Y (2020) miR-26a mediates LC-PUFA biosynthesis by targeting the Lxrα-Srebp1 pathway in the marine teleost Siganus canaliculatus. Journal of Biological Chemistry, 295 (40), pp. 13875-13886. https://doi.org/10.1074/jbc.RA120.014858
Article
Broughton R, Tocher DR & Betancor MB (2020) Development of a C18 Supercritical Fluid Chromatography-Tandem Mass Spectrometry Methodology for the Analysis of Very-Long-Chain Polyunsaturated Fatty Acid Lipid Matrices and Its Application to Fish Oil Substitutes Derived from Genetically Modified Oilseeds in the Aquaculture Sector. ACS Omega, 5 (35), pp. 22289-22298. https://doi.org/10.1021/acsomega.0c02631
Article
Nácher-Mestre J, Beltrán E, Strachan F, Dick JR, Pérez-Sánchez J, Berntssen MHG & Tocher DR (2020) No transfer of the non-regulated mycotoxins, beauvericin and enniatins, from feeds to farmed fish reared on plant-based diets. Food Chemistry, 323, Art. No.: 126773. https://doi.org/10.1016/j.foodchem.2020.126773
Article
Jin M, Zhu T, Tocher D, Luo J, Shen Y, Li X, Pan T, Yuan Y, Betancor M, Jiao L, Sun P & Zhou Q (2020) Dietary fenofibrate attenuated high-fat-diet-induced lipid accumulation and inflammation response partly through regulation of pparα and sirt1 in juvenile black seabream (Acanthopagrus schlegelii). Developmental and Comparative Immunology, 109, Art. No.: 103691. https://doi.org/10.1016/j.dci.2020.103691
Article
Sun J, Chen C, You C, Liu Y, Ma H, Monroig Ó, Tocher DR, Wang S & Li Y (2020) The miR-15/16 Cluster Is Involved in the Regulation of Vertebrate LC-PUFA Biosynthesis by Targeting pparγ as Demonostrated in Rabbitfish Siganus canaliculatus. Marine Biotechnology, 22 (4), p. 475–487. https://doi.org/10.1007/s10126-020-09969-0
Article
Wang X, Jin M, Cheng X, Luo J, Jiao L, Betancor MB, Tocher DR & Zhou Q (2020) Dietary lipid and n-3 LC-PUFA levels impact growth performance and lipid metabolism of juvenile mud crab, Scylla paramamosain. British Journal of Nutrition. https://doi.org/10.1017/s0007114520003335
Article
Luo J, Zhu T, Wang X, Chen X, Yuan Y, Jin M, Betancor M, Tocher D & Zhou Q (2020) Toxicological mechanism of excessive copper supplementation: Effects on coloration, copper bioaccumulation and oxidation resistance in mud crab Scylla paramamosain. Journal of Hazardous Materials, 395, Art. No.: 122600. https://doi.org/10.1016/j.jhazmat.2020.122600
Article
Betancor M, Sprague M, Ortega A, de la Gándara F, Tocher D, Ruth R, Perkins E & Mourente G (2020) Central and peripheral clocks in Atlantic bluefin tuna (Thunnus thynnus, L.): Daily rhythmicity of hepatic lipid metabolism and digestive genes. Aquaculture, 523, Art. No.: 735220. https://doi.org/10.1016/j.aquaculture.2020.735220
Article
Glencross BD, Baily J, Berntssen MHG, Hardy R, MacKenzie S & Tocher DR (2020) Risk assessment of the use of alternative animal and plant raw material resources in aquaculture feeds. Reviews in Aquaculture, 12 (2), pp. 703-758. https://doi.org/10.1111/raq.12347
Article
Goswami RK, Shrivastav AK, Sharma JG, Tocher DR & Chakrabarti R (2020) Growth and digestive enzyme activities of rohu labeo rohita fed diets containing macrophytes and almond oil-cake. Animal Feed Science and Technology, 263, Art. No.: 114456. https://doi.org/10.1016/j.anifeedsci.2020.114456
Article
Li Y, Wen Z, You C, Xie Z, Tocher DR, Zhang Y, Wang S & Li Y (2020) Genome wide identification and functional characterization of two LC-PUFA biosynthesis elongase (elovl8) genes in rabbitfish (Siganus canaliculatus). Aquaculture, 522, Art. No.: 735127. https://doi.org/10.1016/j.aquaculture.2020.735127
Article
Yuan Y, Wang X, Jin M, Jiao L, Sun P, Betancor MB, Tocher DR & Zhou Q (2020) Modification of nutritional values and flavor qualities of muscle of swimming crab (Portunus trituberculatus): Application of a dietary lipid nutrition strategy. Food Chemistry, 308 p. 11, Art. No.: 125607. https://doi.org/10.1016/j.foodchem.2019.125607
Article
Hamre K, Bjørnevik M, Espe M, Conceição LEC, Johansen J, Silva J, Hillestad M, Prabhu AJ, Taylor JF, Tocher DR, Lock E & Hemre G (2020) Dietary micronutrient composition affects fillet texture and muscle cell size in Atlantic salmon (Salmo salar). Aquaculture Nutrition. https://doi.org/10.1111/anu.13051
Article
Betancor M, Oboh A, Ortega A, Mourente G, Navarro JC, de la Gandara F, Tocher D & Monroig O (2020) Molecular and functional characterisation of a putative elovl4 gene and its expression in response to dietary fatty acid profile in Atlantic bluefin tuna (Thunnus thynnus). Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics, 240, Art. No.: 110372. https://doi.org/10.1016/j.cbpb.2019.110372
Article
Xu W, Wang S, You C, Zhang Y, Monroig O, Tocher DR & Li Y (2020) The catadromous teleost Anguilla japonica has a complete enzymatic repertoire for the biosynthesis of docosahexaenoic acid from alpha-linolenic acid: Cloning and functional characterization of an Elovl2 elongase. Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 240, Art. No.: 110373. https://doi.org/10.1016/j.cbpb.2019.110373
Article
Wang S, Wang M, Zhanga H, Yana X, Guoa H, You C, Tocher DR, Chen C & Li Y (2020) Long-chain polyunsaturated fatty acid metabolism in carnivorous marine teleosts: insight into the profile of endogenous biosynthesis in golden pompano Trachinotus ovatus. Aquaculture Research, 51 (2), pp. 623-635. https://doi.org/10.1111/are.14410
Article
Jin M, Pan T, Tocher D, Betancor M, Monroig O, Shen Y, Zhu T, Sun P, Jiao L & Zhou Q (2019) Dietary choline supplementation attenuated high-fat diet-induced inflammation through regulation of lipid metabolism and suppression of NFKB activation in juvenile black seabream (Acanthopagrus schlegelii). Journal of Nutritional Science, 8, Art. No.: e38. https://doi.org/10.1017/jns.2019.34
Article
Vera LM, Lock E, Hamre K, Migaud H, Leeming D, Tocher DR & Taylor JF (2019) Enhanced micronutrient supplementation in low marine diets reduced vertebral malformation in diploid and triploid Atlantic salmon (Salmo salar) parr, and increased vertebral expression of bone biomarker genes in diploids. Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 237, Art. No.: 110327. https://doi.org/10.1016/j.cbpb.2019.110327
Article
Sprague M, Xu G, Betancor MB, Olsen RE, Torrissen O, Glencross BD & Tocher DR (2019) Endogenous production of n-3 long-chain PUFA from first feeding and the influence of dietary linoleic acid and the α-linolenic:linoleic ratio in Atlantic salmon (Salmo salar). British Journal of Nutrition, 122 (10), pp. 1090-1102. https://doi.org/10.1017/S0007114519001946
Article
Sharma JG, Clark WD, Shrivastav AK, Goswami RK, Tocher DR & Chakrabarti R (2019) Production potential of greater duckweed Spirodela polyrhiza (L. Schleiden) and its biochemical composition evaluation. Aquaculture, 513, Art. No.: 734419. https://doi.org/10.1016/j.aquaculture.2019.734419
Article
Li Y, Zhao J, Dong Y, Yin Z, Li Y, Liu Y, You C, Monroig Ó, Tocher DR & Wang S (2019) Sp1 is Involved in Vertebrate LC-PUFA Biosynthesis by Upregulating the Expression of Liver Desaturase and Elongase Genes. International Journal of Molecular Sciences, 20 (20), Art. No.: 5066. https://doi.org/10.3390/ijms20205066
Article
Sun P, Jin M, Jiao L, Monroig O, Navarro JC, Tocher DR, Betancor MB, Wang X, Yuan Y & Zhou Q (2019) Effects of dietary lipid level on growth, fatty acid profiles, antioxidant capacity and expression of genes involved in lipid metabolism in juvenile swimming crab, Portunus trituberculatus. British Journal of Nutrition p. 36. https://doi.org/10.1017/s0007114519002563
Article
Ran Z, Xu J, Liao K, Monroig Ó, Navarro JC, Oboh A, Jin M, Zhou Q, Zhou C, Tocher DR & Yan X (2019) Biosynthesis of long-chain polyunsaturated fatty acids in the razor clam Sinonovacula constricta: characterization of four fatty acyl elongases and a novel desaturase capacity. Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids, 1864 (8), pp. 1083-1090. https://doi.org/10.1016/j.bbalip.2019.04.004
Article
Sun JJ, Zheng LG, Chen CY, Zhang JY, You CH, Zhang QH, Ma HY, Monroig O, Tocher DR, Wang SQ & Li YY (2019) MicroRNAs Involved in the Regulation of LC-PUFA Biosynthesis in Teleosts: miR-33 Enhances LC-PUFA Biosynthesis in Siganus canaliculatus by Targeting insig1 which in Turn Upregulates srebp1. Marine Biotechnology, 21 (4), pp. 475-487. https://doi.org/10.1007/s10126-019-09895-w
Article
Garrido D, Kabeya N, Betancor MB, Perez JA, Acosta NG, Tocher DR, Rodriguez C & Monroig O (2019) Functional diversification of teleost Fads2 fatty acyl desaturases occurs independently of the trophic level. Scientific Reports, 9, Art. No.: 11199. https://doi.org/10.1038/s41598-019-47709-0
Article
Betancor M, Laurent G, Ortega A, de la Gándara F, Tocher D & Mourente G (2019) Taurine metabolism and effects of inclusion levels in rotifer (Brachionus rotundiformis, Tschugunoff, 1921) on Atlantic bluefin tuna (Thunnus thynnus, L.) larvae. Aquaculture, 510, pp. 353-363. https://doi.org/10.1016/j.aquaculture.2019.05.040
Article
Chen C, Wang S, Zhang M, Chen B, You C, Xie D, Liu Y, Monroig Ó, Tocher DR, Waiho K & Li Y (2019) miR-24 is involved in vertebrate LC-PUFA biosynthesis as demonstrated in marine teleost Siganus canaliculatus. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1864 (5), pp. 619-628. https://doi.org/10.1016/j.bbalip.2019.01.010
Article
Betancor MB, Ortega A, de la Gandara F, Tocher DR & Mourente G (2019) Performance, feed utilization, and hepatic metabolic response of weaned juvenile Atlantic bluefin tuna (Thunnus thynnus L.): effects of dietary lipid level and source. Fish Physiology and Biochemistry, 45 (2), pp. 697-718. https://doi.org/10.1007/s10695-018-0587-9
Article
Update on GM canola crops as novel sources of omega-3 fish oils
Napier JA, Olsen R & Tocher DR (2019) Update on GM canola crops as novel sources of omega-3 fish oils. Plant Biotechnology Journal, 17 (4), pp. 703-705. https://doi.org/10.1111/pbi.13045
Article
Evaluation of different feeding protocols for larvae of Atlantic bluefin tuna (Thunnus thynnus L.)
Betancor M, Ortega A, de la Gándara F, Varela JL, Tocher DR & Mourente G (2019) Evaluation of different feeding protocols for larvae of Atlantic bluefin tuna (Thunnus thynnus L.). Aquaculture, 505, pp. 523-538. https://doi.org/10.1016/j.aquaculture.2019.02.063
Article
Taylor JF, Vera LM, De Santis C, Lock E, Espe M, Skjaerven KH, Leeming D, del Pozo J, Mota-Velasco J, Migaud H, Hamre K & Tocher DR (2019) The effect of micronutrient supplementation on growth and hepatic metabolism in diploid and triploid Atlantic salmon (Salmo salar) parr fed a low marine ingredient diet. Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 227, pp. 106-121. https://doi.org/10.1016/j.cbpb.2018.10.004
Article
Zhou W, Rahimnejad S, Tocher DR, Lu K, Zhang C & Sun Y (2019) Metformin attenuates lipid accumulation in hepatocytes of blunt snout bream (Megalobrama amblycephala) via activation of AMP-activated protein kinase. Aquaculture, 499, pp. 90-100. https://doi.org/10.1016/j.aquaculture.2018.09.028
Article
Tocher D, Betancor M, Sprague M, Olsen R & Napier J (2019) Omega-3 Long-Chain Polyunsaturated Fatty Acids, EPA and DHA: Bridging the Gap between Supply and Demand. Nutrients, 11 (1), Art. No.: 89. https://doi.org/10.3390/nu11010089
Article
Betancor M, Olsen RE, Marandel L, Skulstad OF, Madaro A, Tocher DR & Panserat S (2018) Impact of dietary carbohydrate/protein ratio on hepatic metabolism in land-locked Atlantic salmon (Salmo salar L.) [Dietary carbohydrate in land-locked salmon]. Frontiers in Physiology, 9, Art. No.: 1751. https://doi.org/10.3389/fphys.2018.01751
Article
Sprague M, Cooper S, Tocher DR & Betancor MB (2018) Encapsulated fish oil products available in the UK meet regulatory guidelines with respect to EPA + DHA contents and oxidative status. European Journal of Lipid Science and Technology, 120 (10), Art. No.: 1800105. https://doi.org/10.1002/ejlt.201800105
Article
Lopes-Marques M, Kabeya N, Qian Y, Ruivo R, Santos M, Venkatesh B, Tocher DR, Castro LFC & Monroig O (2018) Retention of fatty acyl desaturase 1 (fads1) in Elopomorpha and Cyclostomata provides novel insights into the evolution of long-chain polyunsaturated fatty acid biosynthesis in vertebrates. BMC Evolutionary Biology, 18, Art. No.: 157. https://doi.org/10.1186/s12862-018-1271-5
Article
Mass Production of Lemna minor and Its Amino Acid and Fatty Acid Profiles
Chakrabarti R, Clark WD, Sharma JG, Goswami RK, Shrivastav AK & Tocher DR (2018) Mass Production of Lemna minor and Its Amino Acid and Fatty Acid Profiles. Frontiers in Chemistry, 6, Art. No.: 479. https://doi.org/10.3389/fchem.2018.00479
Article
Li Y, Yin Z, Dong Y, Wang S, Monroig Ó, Tocher DR & You C (2018) Pparγ Is Involved in the Transcriptional Regulation of Liver LC-PUFA Biosynthesis by Targeting the Δ6Δ5 Fatty Acyl Desaturase Gene in the Marine Teleost Siganus canaliculatus. Marine Biotechnology. https://doi.org/10.1007/s10126-018-9854-0
Article
Betancor M, Li K, Bucerzan V, Sprague M, Sayanova O, Usher S, Han L, Norambuena F, Torrisen O, Napier JA, Tocher DR & Olsen RE (2018) Oil from transgenic Camelina sativa containing over 25 % n-3 long-chain polyunsaturated fatty acids as the major lipid source in feed for Atlantic salmon (Salmo salar). British Journal of Nutrition, 119 (12), pp. 1378-1392. https://doi.org/10.1017/S0007114518001125
Article
Wang S, Chen J, Jiang D, Zhang Q, You C, Tocher DR, Monroig O, Dong Y & Li Y (2018) Hnf4α is involved in the regulation of vertebrate LC-PUFA biosynthesis: insights into the regulatory role of Hnf4α on expression of liver fatty acyl desaturases in the marine teleost Siganus canaliculatus. Fish Physiology and Biochemistry, 44 (3), pp. 805-815. https://doi.org/10.1007/s10695-018-0470-8
Article
Genes for de novo biosynthesis of omega-3 polyunsaturated fatty acids are widespread in animals
Kabeya N, Fonseca MM, Ferrier DEK, Navarro JC, Bay LK, Francis DS, Tocher DR, Costa Castro LF & Monroig Ó (2018) Genes for de novo biosynthesis of omega-3 polyunsaturated fatty acids are widespread in animals. Science Advances, 4 (5), Art. No.: eaar6849. https://doi.org/10.1126/sciadv.aar6849
Article
Dong Y, Zhao J, Chen J, Wang S, Liu Y, Zhang Q, You C, Monroig O, Tocher DR & Li Y (2018) Cloning and characterization of ∆6/∆5 fatty acyl desaturase (Fad) gene promoter in the marine teleost Siganus canaliculatus. Gene, 647, pp. 174-180. https://doi.org/10.1016/j.gene.2018.01.031
Article
Xie D, Fu Z, Wang S, You C, Monroig O, Tocher DR & Li Y (2018) Characteristics of the fads2 gene promoter in marine teleost Epinephelus coioides and role of Sp1-binding site in determining promoter activity. Scientific Reports, 8, Art. No.: 5305. https://doi.org/10.1038/s41598-018-23668-w
Article
Kabeya N, Yevzelman S, Oboh A, Tocher DR & Monroig O (2018) Essential fatty acid metabolism and requirements of the cleaner fish, ballan wrasse Labrus bergylta: Defining pathways of long-chain polyunsaturated fatty acid biosynthesis. Aquaculture, 488, pp. 199-206. https://doi.org/10.1016/j.aquaculture.2018.01.039
Article
Houston S, Karalazos V, Tinsley J, Betancor M, Martin SAM, Tocher DR & Monroig O (2017) The compositional and metabolic responses of gilthead seabream (Sparus aurata) to a gradient of dietary fish oil and associated n-3 long-chain polyunsaturated fatty acid content. British Journal of Nutrition, 118 (12), pp. 1010-1022. https://doi.org/10.1017/S0007114517002975
Article
Microbial and genetically engineered oils as replacements for fish oil in aquaculture feeds
Sprague M, Betancor MB & Tocher DR (2017) Microbial and genetically engineered oils as replacements for fish oil in aquaculture feeds. Biotechnology Letters, 39 (11), pp. 1599-1609. https://doi.org/10.1007/s10529-017-2402-6
Article
Vera L, Metochis C, Taylor J, Clarkson M, Skjaerven KH, Migaud H & Tocher DR (2017) Early nutritional programming affects liver transcriptome in diploid and triploid Atlantic salmon, Salmo salar. BMC Genomics, 18 (1), Art. No.: 886. https://doi.org/10.1186/s12864-017-4264-7
Article
Oboh A, Navarro JC, Tocher DR & Monroig O (2017) Elongation of very long-chain (>C24) fatty acids in Clarias gariepinus: Cloning, functional characterization and tissue expression of elovl4 elongases. Lipids, 52 (10), pp. 837-848. https://doi.org/10.1007/s11745-017-4289-3
Article
Jin M, Monroig O, Navarro JC, Tocher DR & Zhou Q (2017) Molecular and functional characterisation of two elovl4 elongases involved in the biosynthesis of very long-chain (>C24) polyunsaturated fatty acids in black seabream Acanthopagrus schlegelii. Comparative Biochemistry and Physiology - B: Comparative Biochemistry, 212, pp. 41-50. https://doi.org/10.1016/j.cbpb.2017.06.008
Article
Betancor M, Ortega A, de la Gandara F, Tocher DR & Mourente G (2017) Molecular aspects of lipid metabolism, digestibility and antioxidant status of Atlantic bluefin tuna (T. thynnus L.) larvae during first feeding. Aquaculture, 479, pp. 357-369. https://doi.org/10.1016/j.aquaculture.2017.06.011
Article
Clarkson M, Migaud H, Metochis C, Vera L, Leeming D, Tocher DR & Taylor J (2017) Early nutritional intervention can improve utilisation of vegetable-based diets in diploid and triploid Atlantic salmon (Salmo salar L.). British Journal of Nutrition, 118 (1), pp. 17-29. https://doi.org/10.1017/S0007114517001842
Meeting Abstract
Sprague M, Betancor M, Dick JR & Tocher DR (2017) Nutritional evaluation of seafood, with respect to long-chain omega-3 fatty acids, available to UK consumers. Nutrition Society Spring Conference 2017: Nutrition and exercise for health and performance, Stirling, 28/03/2017 - 29/03/2017. Proceedings of the Nutrition Society, 76 (OCE2), p. E38. https://www.cambridge.org/core/journals/proceedings-of-the-nutrition-society/article/nutritional-evaluation-of-seafood-with-respect-to-longchain-omega3-fatty-acids-available-to-uk-consumers/81A58EB320FD82BA230C54AEB88D4539; https://doi.org/10.1017/S0029665117000945
Article
Oboh A, Kabeya N, Carmona-Antoñanzas G, Castro LFC, Dick JR, Tocher DR & Monroig O (2017) Two alternative pathways for docosahexaenoic acid (DHA, 22:6n-3) biosynthesis are widespread among teleost fish. Scientific Reports, 7 (1), Art. No.: 3889. https://doi.org/10.1038/s41598-017-04288-2
Article
Wang S, Liu X, Xu S, Wu Q, You C, Monroig O, Tocher DR & Li Y (2017) Total Replacement of Dietary Fish Oil with a Blend of Vegetable Oils in the Marine Herbivorous Teleost, Siganus canaliculatus. Journal of the World Aquaculture Society. https://doi.org/10.1111/jwas.12434
Article
Betancor M, Sprague M, Montero D, Usher S, Sayanova O, Campbell P, Napier JA, Caballero MJ, Izquierdo MS & Tocher DR (2017) Erratum to: Replacement of Marine Fish Oil with de novo Omega-3 Oils from Transgenic Camelina sativa in Feeds for Gilthead Sea Bream (Sparus aurata L.). Lipids, 52 (5), p. 463. https://doi.org/10.1007/s11745-017-4248-z
Article
Li S, Monroig O, Wang T, Yuan Y, Navarro JC, Hontoria F, Liao K, Tocher DR, Mai K, Xu W & Ai Q (2017) Functional characterization and differential nutritional regulation of putative Elovl5 and Elovl4 elongases in large yellow croaker (Larimichthys crocea). Scientific Reports, 7, Art. No.: 2303. https://doi.org/10.1038/s41598-017-02646-8
Article
Betancor M, Ortega A, de la Gandara F, Tocher DR & Mourente G (2017) Lipid metabolism-related gene expression pattern of Atlantic bluefin tuna (Thunnus thynnus L.) larvae fed on live prey. Fish Physiology and Biochemistry, 43 (2), pp. 493-516. https://doi.org/10.1007/s10695-016-0305-4
Article
Jin M, Monroig O, Lu Y, Yuan Y, Ding L, Tocher DR & Zhou Q (2017) Dietary DHA/EPA ratio affected tissue fatty acid profiles, antioxidant capacity, hematological characteristics and expression of lipid-related genes but not growth in juvenile black seabream (Acanthopagrus schlegelii). PLoS ONE, 12 (4), Art. No.: e0176216. https://doi.org/10.1371/journal.pone.0176216
Article
Betancor M, Li K, Sprague M, Bardal T, Sayanova O, Usher S, Han L, Masoval K, Torrissen O, Napier JA, Tocher DR & Olsen RE (2017) An oil containing EPA and DHA from transgenic Camelina sativa to replace marine fish oil in feeds for Atlantic salmon (Salmo salar L.): Effects on intestinal transcriptome, histology, tissue fatty acid profiles and plasma biochemistry. PLoS ONE, 12 (4), Art. No.: e0175415. https://doi.org/10.1371/journal.pone.0175415
Article
Monroig O, de Llanos R, Varo I, Hontoria F, Tocher DR, Puig S & Navarro JC (2017) Biosynthesis of Polyunsaturated Fatty Acids in Octopus vulgaris: Molecular Cloning and Functional Characterisation of a Stearoyl-CoA Desaturase and an Elongation of Very Long-Chain Fatty Acid 4 Protein. Marine Drugs, 15 (3), Art. No.: 82. https://doi.org/10.3390/md15030082
Article
Li S, Monroig Ó, Navarro JC, Yuan Y, Xu W, Mai K, Tocher DR & Ai Q (2017) Molecular Cloning and functional characterization of a putative Elovl4 gene and its expression in response to dietary fatty acid profiles in orange-spotted grouper Epinephelus coioides. Aquaculture Research, 48 (2), pp. 537-552. https://doi.org/10.1111/are.12902
Article
Reis DB, Acosta NG, Almansa E, Navarro JC, Tocher DR, Andrade JP, Sykes AV & Rodriguez C (2017) Comparative study on fatty acid metabolism of early stages of two crustacean species: Artemia sp. metanauplii and Grapsus adscensionis zoeae, as live prey for marine animals. Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 204, pp. 53-60. https://doi.org/10.1016/j.cbpb.2016.11.002
Article
Lopes-Marques M, Ozório R, Amaral R, Tocher DR, Monroig O & Costa Castro LF (2017) Molecular and functional characterization of a fads2 orthologue in the Amazonian teleost, Arapaima gigas. Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 203, pp. 84-91. https://doi.org/10.1016/j.cbpb.2016.09.007
Article
Shepherd CJ, Monroig O & Tocher DR (2017) Future availability of raw materials for salmon feeds and supply chain implications: the case of Scottish farmed salmon. Aquaculture, 467, pp. 49-62. https://doi.org/10.1016/j.aquaculture.2016.08.021
Article
De Santis C, Martin SAM, Dehler CE, Iannetta PPM, Leeming D & Tocher DR (2016) Influence of dietary inclusion of a wet processed faba bean protein isolate on post-smolt Atlantic salmon (Salmo salar). Aquaculture, 465, pp. 124-133. https://doi.org/10.1016/j.aquaculture.2016.09.008
Article
Pike I & Tocher DR (2016) Could an El Niño event put dietary supplies of n-3 long-chain polyunsaturated fatty acids (EPA and DHA) in jeopardy. European Journal of Lipid Science and Technology, 118 (11), pp. 1684-1691. https://doi.org/10.1002/ejlt.201400647
Article
Betancor M, Sprague M, Montero D, Usher S, Sayanova O, Campbell P, Napier JA, Caballero MJ, Izquierdo MS & Tocher DR (2016) Replacement of Marine Fish Oil with de novo Omega-3 Oils from Transgenic Camelina sativa in Feeds for Gilthead Sea Bream (Sparus aurata L.). Lipids, 51 (10), pp. 1171-1191. https://doi.org/10.1007/s11745-016-4191-4
Article
Composition and metabolism of phospholipids in Octopus vulgaris and Sepia officinalis hatchlings
Reis DB, Acosta NG, Almansa E, Tocher DR, Andrade JP, Sykes AV & Rodriguez C (2016) Composition and metabolism of phospholipids in Octopus vulgaris and Sepia officinalis hatchlings. Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 200, pp. 62-68. https://doi.org/10.1016/j.cbpb.2016.06.001
Article
Mellery J, Geay F, Tocher DR, Kestemont P, Debier C, Rollin X & Larondelle Y (2016) Temperature increase negatively affects the fatty acid bioconversion capacity of rainbow trout (Oncorhynchus mykiss) fed a linseed oil-based diet. PLoS ONE, 11 (10), Art. No.: e0164478. https://doi.org/10.1371/journal.pone.0164478
Article
Carmona-Antonanzas G, Zheng X, Tocher DR & Leaver M (2016) Regulatory divergence of homeologous Atlantic salmon elovl5 genes following the salmonid-specific whole genome duplication. Gene, 591 (1), pp. 34-42. https://doi.org/10.1016/j.gene.2016.06.056
Article
Zhang Q, You C, Liu F, Zhu W, Wang S, Xie D, Monroig O, Tocher DR & Li Y (2016) Cloning and characterization of Lxr and Srebp1, and their potential roles in regulation of LC-PUFA biosynthesis in rabbitfish Siganus canaliculatus. Lipids, 51 (9), pp. 1051-1063. https://doi.org/10.1007/s11745-016-4176-3
Article
Zhang Q, You C, Wang S, Dong Y, Monroig O, Tocher DR & Li Y (2016) The miR-33 gene is identified in a marine teleost: a potential role in regulation of LC-PUFA biosynthesis in Siganus canaliculatus. Scientific Reports, 6, Art. No.: 32909. https://doi.org/10.1038/srep32909
Article
Oboh A, Betancor MB, Tocher DR & Monroig O (2016) Biosynthesis of long-chain polyunsaturated fatty acids in the African catfish Clarias gariepinus: Molecular cloning and functional characterisation of fatty acyl desaturase (fads2) and elongase (elovl2) cDNAs7. Aquaculture, 462, pp. 70-79. https://doi.org/10.1016/j.aquaculture.2016.05.018
Article
Xie D, Chen F, Lin S, You C, Wang S, Zhang Q, Monroig O, Tocher DR & Li Y (2016) Long-chain polyunsaturated fatty acid biosynthesis in the euryhaline herbivorous teleost Scatophagus argus: Functional characterization, tissue expression and nutritional regulation of two fatty acyl elongases. Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 198, pp. 37-45. https://doi.org/10.1016/j.cbpb.2016.03.009
Article
Dong Y, Wang S, Chen F, Liu F, You C, Monroig O, Tocher DR & Li Y (2016) Hepatocyte Nuclear Factor 4α (HNF4α) Is a Transcription Factor of Vertebrate Fatty Acyl Desaturase Gene as Identified in Marine Teleost Siganus canaliculatus. PLoS ONE, 11 (7), Art. No.: e0160361. https://doi.org/10.1371/journal.pone.0160361
Article
Betancor M, Sprague M, Sayanova O, Usher S, Metochis C, Campbell P, Napier JA & Tocher DR (2016) Nutritional evaluation of an EPA-DHA oil from transgenic Camelina sativa in feeds for post-smolt Atlantic salmon (Salmo salar L.). PLoS ONE, 11 (7), Art. No.: e0159934. https://doi.org/10.1371/journal.pone.0159934
Article
Costa Castro LF, Tocher DR & Monroig O (2016) Long-chain polyunsaturated fatty acid biosynthesis in chordates: insights into the evolution of Fads and Elovl gene repertoire. Progress in Lipid Research, 62, pp. 25-40. https://doi.org/10.1016/j.plipres.2016.01.001
Article
Betancor M, Dam TMC, Walton J, Morken T, Campbell P & Tocher DR (2016) Modulation of selenium tissue distribution and selenoproteins expression in Atlantic salmon (Salmo salar L.) fed diets with graded levels of plant ingredients. British Journal of Nutrition, 115 (8), pp. 1325-1338. https://doi.org/10.1017/S0007114516000416
Article
Betancor M, Olsen RE, Solstorm D, Skulstad OF & Tocher DR (2016) Assessment of a land-locked Atlantic salmon (Salmo salar L.) population as a potential genetic resource with a focus on long-chain polyunsaturated fatty acid biosynthesis. Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1861 (3), pp. 227-238. https://doi.org/10.1016/j.bbalip.2015.12.015
Article
Abdul Hamid NK, Carmona-Antonanzas G, Monroig O, Tocher DR, Turchini GM & Donald J (2016) Isolation and functional characterisation of a fads2 in rainbow trout (Oncorhynchus mykiss) with ∆5 desaturase activity. PLoS ONE, 11 (3), Art. No.: e0150770. https://doi.org/10.1371/journal.pone.0150770
Article
Differential responses of the gut transcriptome to plant protein diets in farmed Atlantic salmon
Krol E, Douglas A, Tocher DR, Crampton VO, Speakman JR, Secombes CJ & Martin SAM (2016) Differential responses of the gut transcriptome to plant protein diets in farmed Atlantic salmon. BMC Genomics, 17, Art. No.: 156. https://doi.org/10.1186/s12864-016-2473-0
Article
Sprague M, Dick JR & Tocher DR (2016) Impact of sustainable feeds on omega-3 long-chain fatty acid levels in farmed Atlantic salmon, 2006–2015. Scientific Reports, 6, Art. No.: 21892. https://doi.org/10.1038/srep21892
Article
Nutritional regulation of long-chain PUFA biosynthetic genes in rainbow trout (Oncorhynchus mykiss)
Gregory M, Collins RO, Tocher DR, James M & Turchini GM (2016) Nutritional regulation of long-chain PUFA biosynthetic genes in rainbow trout (Oncorhynchus mykiss). British Journal of Nutrition, 115, pp. 1721-1729. https://doi.org/10.1017/S0007114516000830
Article
Evolutionary functional elaboration of the Elovl2/5 gene family in chordates
Monroig O, Lopes-Marques M, Navarro JC, Hontoria F, Ruvio R, Santos M, Venkatesh B, Tocher DR & Costa Castro LF (2016) Evolutionary functional elaboration of the Elovl2/5 gene family in chordates. Scientific Reports, 6, Art. No.: 20510. https://doi.org/10.1038/srep20510
Article
De Santis C, Tocher DR, Ruohonen K, El-Mowafi A, Martin SAM, Dehler C, Secombes CJ & Crampton V (2016) Air-classified faba bean protein concentrate is efficiently utilized as a dietary protein source by post-smolt Atlantic salmon (Salmo salar). Aquaculture, 452, pp. 169-177. https://doi.org/10.1016/j.aquaculture.2015.10.035
Article
Tejera N, Vauzour D, Betancor M, Sayanova O, Usher S, Cochard M, Rigby N, Ruiz-Lopez N, Menoyo D, Tocher DR, Napier JA & Minihane AM (2016) A transgenic Camelina sativa seed oil effectively replaces fish oil as a dietary source of eicosapentaenoic acid in mice. Journal of Nutrition, 146 (2), pp. 227-235. https://doi.org/10.3945/jn.115.223941
Article
In vivo metabolism of unsaturated fatty acids in Sepia officinalis hatchlings
Reis DB, Rodriguez C, Acosta NG, Almansa E, Tocher DR, Andrade JP & Sykes AV (2016) In vivo metabolism of unsaturated fatty acids in Sepia officinalis hatchlings. Aquaculture, 450, pp. 67-73. https://doi.org/10.1016/j.aquaculture.2015.07.012
Article
Monroig O, Hontoria F, Varo I, Tocher DR & Navarro JC (2016) Investigating the essential fatty acids in the common cuttlefish Sepia officinalis (Mollusca, Cephalopoda): Molecular cloning and functional characterisation of fatty acyl desaturase and elongase. Aquaculture, 450, pp. 38-47. https://doi.org/10.1016/j.aquaculture.2015.07.003
