Investigating the potential of nutritional programming to improve the utilisation of sustainable feeds in aquaculture
PI: Professor Douglas Tocher
Funded by: Biomar Ltd and Biotechnology and Biological Sciences Research Council
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Professor Douglas Tocher
Professor
Institute of Aquaculture Pathfoot Building, University of Stirling, Stirling, FK9 4LA
About me
Professor Douglas R. Tocher has a BSc (1979) and PhD (1982) in Biochemistry from the University of Edinburgh, Scotland. He worked for 16 years for the UK Natural Environment Research Council (NERC) at the Institute of Marine Biochemistry, Aberdeen (1982-1986) and the Unit of Aquatic Biochemistry, University of Stirling (1986-1998). His main research interests during this period were fish lipid and fatty acid metabolism including embryonic and early larval development, and cell culture studies on eicosanoid metabolism, polyunsaturated fatty acid synthesis and neural development. In 1998 he joined the Nutrition Group, Institute of Aquaculture, University of Stirling as senior lecturer and was awarded his personal chair in 2009. His current primary research interests are the molecular biology and genetic basis of regulation of lipid and fatty acid metabolism and nutrition in fish, with particular focus on omega-3 (n-3) long-chain polyunsaturated fatty acids. Professor Tocher has co-authored over 300 scientific publications.
Event / Presentation
Polyunsaturated fatty-acids in marine and terrestrial food webs. 11th Conference of the European Society for Comparative Physiology and Biochemistry, and 1st Joint Meeting ESCPB - Association Des Physiologistes
European Society for Comparative Physiology and Biochemistry
Research
The work is directed to the research theme "Fish as Food" with the major drivers being sustainabilty of fish farming, while maintenance of health nenefits of fish, and food security. The overarching aim is to develop alternative, more sustainable feeds not dependent upon finite and limited marine resources (fishmeal and fish oil) with a paricular focus on alterantive supplies of omega-3 (n-3) long-chain polyunsaturated fatty acids. Specific aims of current research are focused on the molecular biology of lipids/fatty acids, particularly hepatic microsomal fatty acyl desaturases and polyunsaturated fatty acid elongases and transcription factors and nuclear receptors including peroxisome proliferator activated receptors (PPARs), sterol regulatory element binding proteins (SREBP) and lipid X receptors (LXR) involved in lipid/fatty acid pathway regulation. Application of post-genomic technologies to determine gene expression in various studies of fish lipid metabolism and nutrition including development and utilization of salmon oligonucleotide microarrays and next generation sequencing.
Projects
ARRAINA
PI: Professor Douglas Tocher
Funded by: European Commission
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Evaluating novel plant oilseeds enriched in omega-3 long-chain polyunsaturated fatty acids to support sustainable development of aquaculture
PI: Professor Douglas Tocher
Funded by: Biomar Ltd and Biotechnology and Biological Sciences Research Council
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SAIC Equipment Award
PI: Professor Jimmy Turnbull
Funded by: Scottish Aquaculture Innovation Centre
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Optimising dietary fatty acids and lipids of Atlantic salmon to secure their health and welfare thorugh varying environmental conditions and life stages (Optihealth)
PI: Professor Brett Glencross
Funded by: The Norwegian Seafood Research Fund
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Evaluating novel plant oilseeds enriched in omega-3 long-chain polyunsaturated fatty acids to support sustainable development of aquaculture
PI: Professor Douglas Tocher
Funded by: Biomar Ltd
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Validation of Fourier-transform infrared spectroscopy as a novel trait analysis tool in salmon to quantify Omega-3 fatty acids
PI: Professor Douglas Tocher
Funded by: Biosciences Knowledge Transfer Network
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Lipid Fatty Acid metabolism and Nutrition in Fish
PI: Professor Douglas Tocher
Funded by: Royal Society
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Functional Characterisation of fatty acyl desaturase cDNA from sea bass by heterologous expression in yeast
PI: Professor Douglas Tocher
Funded by: The Carnegie Trust
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COBIAGENE.
PI: Professor Douglas Tocher
Funded by: European Commission
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Oxidation, Lipids, DNA and Mitochondria
PI: Professor Douglas Tocher
Funded by: European Commission
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Development of a Protein-Rich and Starch-Rich Fractions from Faba Beans for Salmon and Terrestrial Animal Production,
PI: Professor Douglas Tocher
Funded by: Technology Strategy Board
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Marie Curie IEF Fishinutrigen
PI: Professor Douglas Tocher
Funded by: European Commission
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Transcriptional control of polyunsaturated fatty acid synthesis in fish
PI: Professor Douglas Tocher
Funded by: Biotechnology and Biological Sciences Research Council
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Study of the desaturases of omega- 3 highly unsaturated fatty acid (HUFA) biosynthesis in the Cobia (Raxhycentron Canadum)
PI:
Funded by: The Royal Society of Edinburgh
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AQUAMAX: Sustainable Aquafeeds to maximise the health benefits of farmed fish for consumers
PI: Professor Gordon Bell
Funded by: European Commission
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New Marine Feed Resources: The Building of a consortium towards exploitation of under-utilised and un-utilised marine resources for diets to farmed fish
PI: Professor Douglas Tocher
Funded by: Institute of Marine Research
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Outputs
Showing 100 of 314 — See all 314 outputs
Article
Vera LM, Lock E, Hamre K, Migaud H, Leeming D, Tocher DR & Taylor JF (2019) Enhanced micronutrient supplementation in low marine diets reduced vertebral malformation in diploid and triploid Atlantic salmon (Salmo salar) parr, and increased vertebral expression of bone biomarker genes in diploids. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. https://doi.org/10.1016/j.cbpb.2019.110327
Article
Ran Z, Xu J, Liao K, Monroig Ó, Navarro JC, Oboh A, Jin M, Zhou Q, Zhou C, Tocher DR & Yan X (2019) Biosynthesis of long-chain polyunsaturated fatty acids in the razor clam Sinonovacula constricta: characterization of four fatty acyl elongases and a novel desaturase capacity. Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids, 1864 (8), pp. 1083-1090. https://doi.org/10.1016/j.bbalip.2019.04.004
Article
Sun JJ, Zheng LG, Chen CY, Zhang JY, You CH, Zhang QH, Ma HY, Monroig O, Tocher DR, Wang SQ & Li YY (2019) MicroRNAs Involved in the Regulation of LC-PUFA Biosynthesis in Teleosts: miR-33 Enhances LC-PUFA Biosynthesis in Siganus canaliculatus by Targeting insig1 which in Turn Upregulates srebp1. Marine Biotechnology, 21 (4), pp. 475-487. https://doi.org/10.1007/s10126-019-09895-w
Article
Garrido D, Kabeya N, Betancor MB, Perez JA, Acosta NG, Tocher DR, Rodriguez C & Monroig O (2019) Functional diversification of teleost Fads2 fatty acyl desaturases occurs independently of the trophic level. Scientific Reports, 9, Art. No.: 11199. https://doi.org/10.1038/s41598-019-47709-0
Article
Betancor M, Laurent G, Ortega A, de la Gándara F, Tocher D & Mourente G (2019) Taurine metabolism and effects of inclusion levels in rotifer (Brachionus rotundiformis, Tschugunoff, 1921) on Atlantic bluefin tuna (Thunnus thynnus, L.) larvae. Aquaculture, 510, pp. 353-363. https://doi.org/10.1016/j.aquaculture.2019.05.040
Article
Chen C, Wang S, Zhang M, Chen B, You C, Xie D, Liu Y, Monroig Ó, Tocher DR, Waiho K & Li Y (2019) miR-24 is involved in vertebrate LC-PUFA biosynthesis as demonstrated in marine teleost Siganus canaliculatus. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1864 (5), pp. 619-628. https://doi.org/10.1016/j.bbalip.2019.01.010
Article
Betancor MB, Ortega A, de la Gandara F, Tocher DR & Mourente G (2019) Performance, feed utilization, and hepatic metabolic response of weaned juvenile Atlantic bluefin tuna (Thunnus thynnus L.): effects of dietary lipid level and source. Fish Physiology and Biochemistry, 45 (2), pp. 697-718. https://doi.org/10.1007/s10695-018-0587-9
Article
Update on GM canola crops as novel sources of omega-3 fish oils
Napier JA, Olsen R & Tocher DR (2019) Update on GM canola crops as novel sources of omega-3 fish oils. Plant Biotechnology Journal, 17 (4), pp. 703-705. https://doi.org/10.1111/pbi.13045
Article
Evaluation of different feeding protocols for larvae of Atlantic bluefin tuna (Thunnus thynnus L.)
Betancor M, Ortega A, de la Gándara F, Varela JL, Tocher DR & Mourente G (2019) Evaluation of different feeding protocols for larvae of Atlantic bluefin tuna (Thunnus thynnus L.). Aquaculture, 505, pp. 523-538. https://doi.org/10.1016/j.aquaculture.2019.02.063
Article
Glencross BD, Baily J, Berntssen MHG, Hardy R, MacKenzie S & Tocher DR (2019) Risk assessment of the use of alternative animal and plant raw material resources in aquaculture feeds. Reviews in Aquaculture. https://doi.org/10.1111/raq.12347
Article
Taylor JF, Vera LM, De Santis C, Lock E, Espe M, Skjaerven KH, Leeming D, del Pozo J, Mota-Velasco J, Migaud H, Hamre K & Tocher DR (2019) The effect of micronutrient supplementation on growth and hepatic metabolism in diploid and triploid Atlantic salmon (Salmo salar) parr fed a low marine ingredient diet. Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 227, pp. 106-121. https://doi.org/10.1016/j.cbpb.2018.10.004
Article
Zhou W, Rahimnejad S, Tocher DR, Lu K, Zhang C & Sun Y (2019) Metformin attenuates lipid accumulation in hepatocytes of blunt snout bream (Megalobrama amblycephala) via activation of AMP-activated protein kinase. Aquaculture, 499, pp. 90-100. https://doi.org/10.1016/j.aquaculture.2018.09.028
Article
Tocher D, Betancor M, Sprague M, Olsen R & Napier J (2019) Omega-3 Long-Chain Polyunsaturated Fatty Acids, EPA and DHA: Bridging the Gap between Supply and Demand. Nutrients, 11 (1), Art. No.: 89. https://doi.org/10.3390/nu11010089
Article
Betancor M, Olsen RE, Marandel L, Skulstad OF, Madaro A, Tocher DR & Panserat S (2018) Impact of dietary carbohydrate/protein ratio on hepatic metabolism in land-locked Atlantic salmon (Salmo salar L.) [Dietary carbohydrate in land-locked salmon]. Frontiers in Physiology, 9, Art. No.: 1751. https://doi.org/10.3389/fphys.2018.01751
Article
Sprague M, Cooper S, Tocher DR & Betancor MB (2018) Encapsulated fish oil products available in the UK meet regulatory guidelines with respect to EPA + DHA contents and oxidative status. European Journal of Lipid Science and Technology, 120 (10), Art. No.: 1800105. https://doi.org/10.1002/ejlt.201800105
Article
Lopes-Marques M, Kabeya N, Qian Y, Ruivo R, Santos M, Venkatesh B, Tocher DR, Castro LFC & Monroig O (2018) Retention of fatty acyl desaturase 1 (fads1) in Elopomorpha and Cyclostomata provides novel insights into the evolution of long-chain polyunsaturated fatty acid biosynthesis in vertebrates. BMC Evolutionary Biology, 18, Art. No.: 157. https://doi.org/10.1186/s12862-018-1271-5
Article
Mass Production of Lemna minor and Its Amino Acid and Fatty Acid Profiles
Chakrabarti R, Clark WD, Sharma JG, Goswami RK, Shrivastav AK & Tocher DR (2018) Mass Production of Lemna minor and Its Amino Acid and Fatty Acid Profiles. Frontiers in Chemistry, 6, Art. No.: 479. https://doi.org/10.3389/fchem.2018.00479
Article
Li Y, Yin Z, Dong Y, Wang S, Monroig Ó, Tocher DR & You C (2018) Pparγ Is Involved in the Transcriptional Regulation of Liver LC-PUFA Biosynthesis by Targeting the Δ6Δ5 Fatty Acyl Desaturase Gene in the Marine Teleost Siganus canaliculatus. Marine Biotechnology. https://doi.org/10.1007/s10126-018-9854-0
Article
Betancor M, Li K, Bucerzan V, Sprague M, Sayanova O, Usher S, Han L, Norambuena F, Torrisen O, Napier JA, Tocher DR & Olsen RE (2018) Oil from transgenic Camelina sativa containing over 25 % n-3 long-chain polyunsaturated fatty acids as the major lipid source in feed for Atlantic salmon (Salmo salar). British Journal of Nutrition, 119 (12), pp. 1378-1392. https://doi.org/10.1017/S0007114518001125
Article
Wang S, Chen J, Jiang D, Zhang Q, You C, Tocher DR, Monroig O, Dong Y & Li Y (2018) Hnf4α is involved in the regulation of vertebrate LC-PUFA biosynthesis: insights into the regulatory role of Hnf4α on expression of liver fatty acyl desaturases in the marine teleost Siganus canaliculatus. Fish Physiology and Biochemistry, 44 (3), pp. 805-815. https://doi.org/10.1007/s10695-018-0470-8
Article
Genes for de novo biosynthesis of omega-3 polyunsaturated fatty acids are widespread in animals
Kabeya N, Fonseca MM, Ferrier DEK, Navarro JC, Bay LK, Francis DS, Tocher DR, Costa Castro LF & Monroig Ó (2018) Genes for de novo biosynthesis of omega-3 polyunsaturated fatty acids are widespread in animals. Science Advances, 4 (5), Art. No.: eaar6849. https://doi.org/10.1126/sciadv.aar6849
Article
Xie D, Fu Z, Wang S, You C, Monroig O, Tocher DR & Li Y (2018) Characteristics of the fads2 gene promoter in marine teleost Epinephelus coioides and role of Sp1-binding site in determining promoter activity. Scientific Reports, 8, Art. No.: 5305. https://doi.org/10.1038/s41598-018-23668-w
Article
Dong Y, Zhao J, Chen J, Wang S, Liu Y, Zhang Q, You C, Monroig O, Tocher DR & Li Y (2018) Cloning and characterization of ∆6/∆5 fatty acyl desaturase (Fad) gene promoter in the marine teleost Siganus canaliculatus. Gene, 647, pp. 174-180. https://doi.org/10.1016/j.gene.2018.01.031
Article
Kabeya N, Yevzelman S, Oboh A, Tocher DR & Monroig O (2018) Essential fatty acid metabolism and requirements of the cleaner fish, ballan wrasse Labrus bergylta: Defining pathways of long-chain polyunsaturated fatty acid biosynthesis. Aquaculture, 488, pp. 199-206. https://doi.org/10.1016/j.aquaculture.2018.01.039
Article
Houston S, Karalazos V, Tinsley J, Betancor M, Martin SAM, Tocher DR & Monroig O (2017) The compositional and metabolic responses of gilthead seabream (Sparus aurata) to a gradient of dietary fish oil and associated n-3 long-chain polyunsaturated fatty acid content. British Journal of Nutrition, 118 (12), pp. 1010-1022. https://doi.org/10.1017/S0007114517002975
Article
Microbial and genetically engineered oils as replacements for fish oil in aquaculture feeds
Sprague M, Betancor MB & Tocher DR (2017) Microbial and genetically engineered oils as replacements for fish oil in aquaculture feeds. Biotechnology Letters, 39 (11), pp. 1599-1609. https://doi.org/10.1007/s10529-017-2402-6
Article
Vera L, Metochis C, Taylor J, Clarkson M, Skjaerven KH, Migaud H & Tocher DR (2017) Early nutritional programming affects liver transcriptome in diploid and triploid Atlantic salmon, Salmo salar. BMC Genomics, 18 (1), Art. No.: 886. https://doi.org/10.1186/s12864-017-4264-7
Article
Oboh A, Navarro JC, Tocher DR & Monroig O (2017) Elongation of very long-chain (>C24) fatty acids in Clarias gariepinus: Cloning, functional characterization and tissue expression of elovl4 elongases. Lipids, 52 (10), pp. 837-848. https://doi.org/10.1007/s11745-017-4289-3
Article
Jin M, Monroig O, Navarro JC, Tocher DR & Zhou Q (2017) Molecular and functional characterisation of two elovl4 elongases involved in the biosynthesis of very long-chain (>C24) polyunsaturated fatty acids in black seabream Acanthopagrus schlegelii. Comparative Biochemistry and Physiology - B: Comparative Biochemistry, 212, pp. 41-50. https://doi.org/10.1016/j.cbpb.2017.06.008
Article
Betancor M, Ortega A, de la Gandara F, Tocher DR & Mourente G (2017) Molecular aspects of lipid metabolism, digestibility and antioxidant status of Atlantic bluefin tuna (T. thynnus L.) larvae during first feeding. Aquaculture, 479, pp. 357-369. https://doi.org/10.1016/j.aquaculture.2017.06.011
Meeting Abstract
Sprague M, Betancor M, Dick JR & Tocher DR (2017) Nutritional evaluation of seafood, with respect to long-chain omega-3 fatty acids, available to UK consumers. Nutrition Society Spring Conference 2017: Nutrition and exercise for health and performance, Stirling, 28/03/2017 - 29/03/2017. Proceedings of the Nutrition Society, 76 (OCE2), p. E38. https://www.cambridge.org/core/journals/proceedings-of-the-nutrition-society/article/nutritional-evaluation-of-seafood-with-respect-to-longchain-omega3-fatty-acids-available-to-uk-consumers/81A58EB320FD82BA230C54AEB88D4539; https://doi.org/10.1017/S0029665117000945
Article
Clarkson M, Migaud H, Metochis C, Vera L, Leeming D, Tocher DR & Taylor J (2017) Early nutritional intervention can improve utilisation of vegetable-based diets in diploid and triploid Atlantic salmon (Salmo salar L.). British Journal of Nutrition. https://doi.org/10.1017/S0007114517001842
Article
Oboh A, Kabeya N, Carmona-Antoñanzas G, Castro LFC, Dick JR, Tocher DR & Monroig O (2017) Two alternative pathways for docosahexaenoic acid (DHA, 22:6n-3) biosynthesis are widespread among teleost fish. Scientific Reports, 7 (1), Art. No.: 3889. https://doi.org/10.1038/s41598-017-04288-2
Article
Wang S, Liu X, Xu S, Wu Q, You C, Monroig O, Tocher DR & Li Y (2017) Total Replacement of Dietary Fish Oil with a Blend of Vegetable Oils in the Marine Herbivorous Teleost, Siganus canaliculatus. Journal of the World Aquaculture Society. https://doi.org/10.1111/jwas.12434
Article
Betancor M, Sprague M, Montero D, Usher S, Sayanova O, Campbell P, Napier JA, Caballero MJ, Izquierdo MS & Tocher DR (2017) Erratum to: Replacement of Marine Fish Oil with de novo Omega-3 Oils from Transgenic Camelina sativa in Feeds for Gilthead Sea Bream (Sparus aurata L.). Lipids, 52 (5), p. 463. https://doi.org/10.1007/s11745-017-4248-z
Article
Li S, Monroig O, Wang T, Yuan Y, Navarro JC, Hontoria F, Liao K, Tocher DR, Mai K, Xu W & Ai Q (2017) Functional characterization and differential nutritional regulation of putative Elovl5 and Elovl4 elongases in large yellow croaker (Larimichthys crocea). Scientific Reports, 7, Art. No.: 2303. https://doi.org/10.1038/s41598-017-02646-8
Article
Betancor M, Ortega A, de la Gandara F, Tocher DR & Mourente G (2017) Lipid metabolism-related gene expression pattern of Atlantic bluefin tuna (Thunnus thynnus L.) larvae fed on live prey. Fish Physiology and Biochemistry, 43 (2), pp. 493-516. https://doi.org/10.1007/s10695-016-0305-4
Article
Jin M, Monroig O, Lu Y, Yuan Y, Ding L, Tocher DR & Zhou Q (2017) Dietary DHA/EPA ratio affected tissue fatty acid profiles, antioxidant capacity, hematological characteristics and expression of lipid-related genes but not growth in juvenile black seabream (Acanthopagrus schlegelii). PLoS ONE, 12 (4), Art. No.: e0176216. https://doi.org/10.1371/journal.pone.0176216
Article
Betancor M, Li K, Sprague M, Bardal T, Sayanova O, Usher S, Han L, Masoval K, Torrissen O, Napier JA, Tocher DR & Olsen RE (2017) An oil containing EPA and DHA from transgenic Camelina sativa to replace marine fish oil in feeds for Atlantic salmon (Salmo salar L.): Effects on intestinal transcriptome, histology, tissue fatty acid profiles and plasma biochemistry. PLoS ONE, 12 (4), Art. No.: e0175415. https://doi.org/10.1371/journal.pone.0175415
Article
Monroig O, de Llanos R, Varo I, Hontoria F, Tocher DR, Puig S & Navarro JC (2017) Biosynthesis of Polyunsaturated Fatty Acids in Octopus vulgaris: Molecular Cloning and Functional Characterisation of a Stearoyl-CoA Desaturase and an Elongation of Very Long-Chain Fatty Acid 4 Protein. Marine Drugs, 15 (3), Art. No.: 82. https://doi.org/10.3390/md15030082
Article
Li S, Monroig Ó, Navarro JC, Yuan Y, Xu W, Mai K, Tocher DR & Ai Q (2017) Molecular Cloning and functional characterization of a putative Elovl4 gene and its expression in response to dietary fatty acid profiles in orange-spotted grouper Epinephelus coioides. Aquaculture Research, 48 (2), pp. 537-552. https://doi.org/10.1111/are.12902
Article
Reis DB, Acosta NG, Almansa E, Navarro JC, Tocher DR, Andrade JP, Sykes AV & Rodriguez C (2017) Comparative study on fatty acid metabolism of early stages of two crustacean species: Artemia sp. metanauplii and Grapsus adscensionis zoeae, as live prey for marine animals. Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 204, pp. 53-60. https://doi.org/10.1016/j.cbpb.2016.11.002
Article
Lopes-Marques M, Ozório R, Amaral R, Tocher DR, Monroig O & Costa Castro LF (2017) Molecular and functional characterization of a fads2 orthologue in the Amazonian teleost, Arapaima gigas. Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 203, pp. 84-91. https://doi.org/10.1016/j.cbpb.2016.09.007
Article
Shepherd CJ, Monroig O & Tocher DR (2017) Future availability of raw materials for salmon feeds and supply chain implications: the case of Scottish farmed salmon. Aquaculture, 467, pp. 49-62. https://doi.org/10.1016/j.aquaculture.2016.08.021
Article
De Santis C, Martin SAM, Dehler CE, Iannetta PPM, Leeming D & Tocher DR (2016) Influence of dietary inclusion of a wet processed faba bean protein isolate on post-smolt Atlantic salmon (Salmo salar). Aquaculture, 465, pp. 124-133. https://doi.org/10.1016/j.aquaculture.2016.09.008
Article
Pike I & Tocher DR (2016) Could an El Niño event put dietary supplies of n-3 long-chain polyunsaturated fatty acids (EPA and DHA) in jeopardy. European Journal of Lipid Science and Technology, 118 (11), pp. 1684-1691. https://doi.org/10.1002/ejlt.201400647
Article
Composition and metabolism of phospholipids in Octopus vulgaris and Sepia officinalis hatchlings
Reis DB, Acosta NG, Almansa E, Tocher DR, Andrade JP, Sykes AV & Rodriguez C (2016) Composition and metabolism of phospholipids in Octopus vulgaris and Sepia officinalis hatchlings. Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 200, pp. 62-68. https://doi.org/10.1016/j.cbpb.2016.06.001
Article
Betancor M, Sprague M, Montero D, Usher S, Sayanova O, Campbell P, Napier JA, Caballero MJ, Izquierdo MS & Tocher DR (2016) Replacement of Marine Fish Oil with de novo Omega-3 Oils from Transgenic Camelina sativa in Feeds for Gilthead Sea Bream (Sparus aurata L.). Lipids, 51 (10), pp. 1171-1191. https://doi.org/10.1007/s11745-016-4191-4
Article
Mellery J, Geay F, Tocher DR, Kestemont P, Debier C, Rollin X & Larondelle Y (2016) Temperature increase negatively affects the fatty acid bioconversion capacity of rainbow trout (Oncorhynchus mykiss) fed a linseed oil-based diet. PLoS ONE, 11 (10), Art. No.: e0164478. https://doi.org/10.1371/journal.pone.0164478
Article
Carmona-Antonanzas G, Zheng X, Tocher DR & Leaver M (2016) Regulatory divergence of homeologous Atlantic salmon elovl5 genes following the salmonid-specific whole genome duplication. Gene, 591 (1), pp. 34-42. https://doi.org/10.1016/j.gene.2016.06.056
Article
Zhang Q, You C, Liu F, Zhu W, Wang S, Xie D, Monroig O, Tocher DR & Li Y (2016) Cloning and characterization of Lxr and Srebp1, and their potential roles in regulation of LC-PUFA biosynthesis in rabbitfish Siganus canaliculatus. Lipids, 51 (9), pp. 1051-1063. https://doi.org/10.1007/s11745-016-4176-3
Article
Zhang Q, You C, Wang S, Dong Y, Monroig O, Tocher DR & Li Y (2016) The miR-33 gene is identified in a marine teleost: a potential role in regulation of LC-PUFA biosynthesis in Siganus canaliculatus. Scientific Reports, 6, Art. No.: 32909. https://doi.org/10.1038/srep32909
Article
Oboh A, Betancor MB, Tocher DR & Monroig O (2016) Biosynthesis of long-chain polyunsaturated fatty acids in the African catfish Clarias gariepinus: Molecular cloning and functional characterisation of fatty acyl desaturase (fads2) and elongase (elovl2) cDNAs7. Aquaculture, 462, pp. 70-79. https://doi.org/10.1016/j.aquaculture.2016.05.018
Article
Xie D, Chen F, Lin S, You C, Wang S, Zhang Q, Monroig O, Tocher DR & Li Y (2016) Long-chain polyunsaturated fatty acid biosynthesis in the euryhaline herbivorous teleost Scatophagus argus: Functional characterization, tissue expression and nutritional regulation of two fatty acyl elongases. Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 198, pp. 37-45. https://doi.org/10.1016/j.cbpb.2016.03.009
Article
Dong Y, Wang S, Chen F, Liu F, You C, Monroig O, Tocher DR & Li Y (2016) Hepatocyte Nuclear Factor 4α (HNF4α) Is a Transcription Factor of Vertebrate Fatty Acyl Desaturase Gene as Identified in Marine Teleost Siganus canaliculatus. PLoS ONE, 11 (7), Art. No.: e0160361. https://doi.org/10.1371/journal.pone.0160361
Article
Betancor M, Sprague M, Sayanova O, Usher S, Metochis C, Campbell P, Napier JA & Tocher DR (2016) Nutritional evaluation of an EPA-DHA oil from transgenic Camelina sativa in feeds for post-smolt Atlantic salmon (Salmo salar L.). PLoS ONE, 11 (7), Art. No.: e0159934. https://doi.org/10.1371/journal.pone.0159934
Article
Costa Castro LF, Tocher DR & Monroig O (2016) Long-chain polyunsaturated fatty acid biosynthesis in chordates: insights into the evolution of Fads and Elovl gene repertoire. Progress in Lipid Research, 62, pp. 25-40. https://doi.org/10.1016/j.plipres.2016.01.001
Article
Betancor M, Dam TMC, Walton J, Morken T, Campbell P & Tocher DR (2016) Modulation of selenium tissue distribution and selenoproteins expression in Atlantic salmon (Salmo salar L.) fed diets with graded levels of plant ingredients. British Journal of Nutrition, 115 (8), pp. 1325-1338. https://doi.org/10.1017/S0007114516000416
Article
Betancor M, Olsen RE, Solstorm D, Skulstad OF & Tocher DR (2016) Assessment of a land-locked Atlantic salmon (Salmo salar L.) population as a potential genetic resource with a focus on long-chain polyunsaturated fatty acid biosynthesis. Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1861 (3), pp. 227-238. https://doi.org/10.1016/j.bbalip.2015.12.015
Article
Abdul Hamid NK, Carmona-Antonanzas G, Monroig O, Tocher DR, Turchini GM & Donald J (2016) Isolation and functional characterisation of a fads2 in rainbow trout (Oncorhynchus mykiss) with ∆5 desaturase activity. PLoS ONE, 11 (3), Art. No.: e0150770. https://doi.org/10.1371/journal.pone.0150770
Article
Differential responses of the gut transcriptome to plant protein diets in farmed Atlantic salmon
Krol E, Douglas A, Tocher DR, Crampton VO, Speakman JR, Secombes CJ & Martin SAM (2016) Differential responses of the gut transcriptome to plant protein diets in farmed Atlantic salmon. BMC Genomics, 17, Art. No.: 156. https://doi.org/10.1186/s12864-016-2473-0
Article
Sprague M, Dick JR & Tocher DR (2016) Impact of sustainable feeds on omega-3 long-chain fatty acid levels in farmed Atlantic salmon, 2006–2015. Scientific Reports, 6, Art. No.: 21892. https://doi.org/10.1038/srep21892
Article
Nutritional regulation of long-chain PUFA biosynthetic genes in rainbow trout (Oncorhynchus mykiss)
Gregory M, Collins RO, Tocher DR, James M & Turchini GM (2016) Nutritional regulation of long-chain PUFA biosynthetic genes in rainbow trout (Oncorhynchus mykiss). British Journal of Nutrition, 115, pp. 1721-1729. https://doi.org/10.1017/S0007114516000830
Article
Evolutionary functional elaboration of the Elovl2/5 gene family in chordates
Monroig O, Lopes-Marques M, Navarro JC, Hontoria F, Ruvio R, Santos M, Venkatesh B, Tocher DR & Costa Castro LF (2016) Evolutionary functional elaboration of the Elovl2/5 gene family in chordates. Scientific Reports, 6, Art. No.: 20510. https://doi.org/10.1038/srep20510
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Tejera N, Vauzour D, Betancor M, Sayanova O, Usher S, Cochard M, Rigby N, Ruiz-Lopez N, Menoyo D, Tocher DR, Napier JA & Minihane AM (2016) A transgenic Camelina sativa seed oil effectively replaces fish oil as a dietary source of eicosapentaenoic acid in mice. Journal of Nutrition, 146 (2), pp. 227-235. https://doi.org/10.3945/jn.115.223941
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De Santis C, Tocher DR, Ruohonen K, El-Mowafi A, Martin SAM, Dehler C, Secombes CJ & Crampton V (2016) Air-classified faba bean protein concentrate is efficiently utilized as a dietary protein source by post-smolt Atlantic salmon (Salmo salar). Aquaculture, 452, pp. 169-177. https://doi.org/10.1016/j.aquaculture.2015.10.035
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In vivo metabolism of unsaturated fatty acids in Sepia officinalis hatchlings
Reis DB, Rodriguez C, Acosta NG, Almansa E, Tocher DR, Andrade JP & Sykes AV (2016) In vivo metabolism of unsaturated fatty acids in Sepia officinalis hatchlings. Aquaculture, 450, pp. 67-73. https://doi.org/10.1016/j.aquaculture.2015.07.012
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Monroig O, Hontoria F, Varo I, Tocher DR & Navarro JC (2016) Investigating the essential fatty acids in the common cuttlefish Sepia officinalis (Mollusca, Cephalopoda): Molecular cloning and functional characterisation of fatty acyl desaturase and elongase. Aquaculture, 450, pp. 38-47. https://doi.org/10.1016/j.aquaculture.2015.07.003
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De Santis C, Crampton V, Bicskei B & Tocher DR (2015) Replacement of dietary soy- with air classified faba bean protein concentrate alters the hepatic transcriptome in Atlantic salmon (Salmo salar) parr. Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics, 16, pp. 48-58. https://doi.org/10.1016/j.cbd.2015.07.005
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Dynamics of fatty acid metabolism in a cell line from southern bluefin tuna (Thunnus maccoyii)
Schofield A, Tocher DR & Schuller KA (2015) Dynamics of fatty acid metabolism in a cell line from southern bluefin tuna (Thunnus maccoyii). Aquaculture, 449, pp. 58-68. https://doi.org/10.1016/j.aquaculture.2015.02.017
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Omega-3 long-chain polyunsaturated fatty acids and aquaculture in perspective
Tocher DR (2015) Omega-3 long-chain polyunsaturated fatty acids and aquaculture in perspective. Aquaculture, 449, pp. 94-107. https://doi.org/10.1016/j.aquaculture.2015.01.010
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Molecular mechanism of dietary phospholipid requirement of Atlantic salmon, Salmo salar, fry
Carmona-Antoñanzas G, Taylor J, Martinez-Rubio L & Tocher DR (2015) Molecular mechanism of dietary phospholipid requirement of Atlantic salmon, Salmo salar, fry. Biochimica et Biophysica Acta (BBA)- Molecular and Cell Biology of Lipids, 1851 (11), pp. 1428-1441. https://doi.org/10.1016/j.bbalip.2015.08.006
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Taylor J, Martinez-Rubio L, del Pozo J, Walton J, Tinch AE, Migaud H & Tocher DR (2015) Influence of dietary phospholipid on early development and performance of Atlantic salmon (Salmo salar). Aquaculture, 448, pp. 262-272. https://doi.org/10.1016/j.aquaculture.2015.06.012
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Betancor M, Almaida P, Hernandez A & Tocher DR (2015) Effects of dietary fatty acids on mitochondrial phospholipid compositions, oxidative status and mitochondrial gene expression of zebrafish at different ages. Fish Physiology and Biochemistry, 41 (5), pp. 1187-1204. https://doi.org/10.1007/s10695-015-0079-0
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Xie D, Wang S, You C, Chen F, Tocher DR & Li Y (2015) Characteristics of LC-PUFA biosynthesis in marine herbivorous teleost Siganus canaliculatus under different ambient salinities. Aquaculture Nutrition, 21 (5), pp. 541-551. https://doi.org/10.1111/anu.12178
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De Santis C, Taylor J, Martinez-Rubio L, Boltana S & Tocher DR (2015) Influence of Development and Dietary Phospholipid Content and Composition on Intestinal Transcriptome of Atlantic Salmon (Salmo salar). PLoS ONE, 10 (10), Art. No.: e0140964. https://doi.org/10.1371/journal.pone.0140964
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De Santis C, Bartie K, Olsen RE, Taggart J & Tocher DR (2015) Nutrigenomic profiling of transcriptional processes affected in liver and distal intestine in response to a soybean meal-induced nutritional stress in Atlantic salmon (Salmo salar). Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics, 15, pp. 1-11. https://doi.org/10.1016/j.cbd.2015.04.001
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The supply of fish oil to aquaculture: a role for transgenic oilseed crops?
Usher S, Haslam R, Sayanova O, Napier JA, Betancor M & Tocher DR (2015) The supply of fish oil to aquaculture: a role for transgenic oilseed crops?. World Agriculture, 5 (1), pp. 15-23.
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Glencross B, De Santis C, Bicskei B, Taggart J, Bron J, Betancor M & Tocher DR (2015) A comparative analysis of the response of the hepatic transcriptome to dietary docosahexaenoic acid in Atlantic salmon (Salmo salar) post-smolts. BMC Genomics, 16 (1), Art. No.: 684. https://doi.org/10.1186/s12864-015-1810-z
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GM crop ban: how Scottish salmon – and public health – could have benefited from this technology
Tocher D (2015) GM crop ban: how Scottish salmon – and public health – could have benefited from this technology. 12.08.2015. https://theconversation.com/gm-crop-ban-how-scottish-salmon-and-public-health-could-have-benefited-from-this-technology-45982
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De Santis C, Ruohonen K, Tocher DR, Martin SAM, Krol E, Secombes CJ, Bell JG, El-Mowafi A & Crampton V (2015) Atlantic salmon (Salmo salar) parr as a model to predict the optimum inclusion of air classified faba bean protein concentrate in feeds for seawater salmon. Aquaculture, 444, pp. 70-78. https://doi.org/10.1016/j.aquaculture.2015.03.024
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Betancor M, Sprague M, Sayanova O, Usher S, Campbell P, Napier JA, Caballero MJ & Tocher DR (2015) Evaluation of a high-EPA oil from transgenic Camelina sativa in feeds for Atlantic salmon (Salmo salar L.): Effects on tissue fatty acid composition, histology and gene expression. Aquaculture, 444, pp. 1-12. https://doi.org/10.1016/j.aquaculture.2015.03.020
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Betancor M, Almaida P, Sprague M, Hernandez A & Tocher DR (2015) Roles of selenoprotein antioxidant protection in zebrafish, Danio rerio, subjected to dietary oxidative stress. Fish Physiology and Biochemistry, 41 (3), pp. 705-720. https://doi.org/10.1007/s10695-015-0040-2
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Morais S, Mourente G, Martinez A, Gras N & Tocher DR (2015) Docosahexaenoic acid biosynthesis via fatty acyl elongase and Δ4-desaturase and its modulation by dietary lipid level and fatty acid composition in a marine vertebrate. Biochimica et Biophysica Acta (BBA)- Molecular and Cell Biology of Lipids, 1851 (5), pp. 588-597. https://doi.org/10.1016/j.bbalip.2015.01.014
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Carboni S, Hughes AD, Atack T, Tocher DR & Migaud H (2015) Influence of broodstock diet on somatic growth, fecundity, gonad carotenoids and larval survival of sea urchin. Aquaculture Research, 46 (4), pp. 969-976. https://doi.org/10.1111/are.12256
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Murray DS, Hager H, Tocher DR & Kainz MJ (2015) Docosahexaenoic acid in Arctic charr (Salvelinus alpinus): The importance of dietary supply and physiological response during the entire growth period. Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 181, pp. 7-14. https://doi.org/10.1016/j.cbpb.2014.11.003
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Almaida P, De Santis C, Rubio-Mejia OL & Tocher DR (2015) Dietary fatty acids affect mitochondrial phospholipid compositions and mitochondrial gene expression of rainbow trout liver at different ages. Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology, 185 (1), pp. 73-86. https://doi.org/10.1007/s00360-014-0870-8
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Betancor M, Sprague M, Usher S, Sayanova O, Campbell P, Napier JA & Tocher DR (2015) A nutritionally-enhanced oil from transgenic Camelina sativa effectively replaces fish oil as a source of eicosapentaenoic acid for fish. Scientific Reports, 5, Art. No.: 8104. https://doi.org/10.1038/srep08104
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Wang S, Monroig O, Tang G, Zhang L, You C, Tocher DR & Li Y (2014) Investigating long-chain polyunsaturated fatty acid biosynthesis in teleost fish: Functional characterization of fatty acyl desaturase (Fads2) and Elovl5 elongase in the catadromous species, Japanese eel Anguilla japonica. Aquaculture, 434, pp. 57-65. https://doi.org/10.1016/j.aquaculture.2014.07.016
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Henriques J, Dick JR, Tocher DR & Bell JG (2014) Nutritional quality of salmon products available from major retailers in the UK: content and composition of n-3 long-chain PUFA. British Journal of Nutrition, 112 (6), pp. 964-975. https://doi.org/10.1017/S0007114514001603
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Daily rhythms in expression of genes of hepatic lipid metabolism in Atlantic salmon (Salmo salar L.)
Betancor M, McStay E, Minghetti M, Migaud H, Tocher DR & Davie A (2014) Daily rhythms in expression of genes of hepatic lipid metabolism in Atlantic salmon (Salmo salar L.). PLoS ONE, 9 (9), Art. No.: e106739. https://doi.org/10.1371/journal.pone.0106739
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Glencross B, Tocher DR, Matthew C & Bell JG (2014) Interactions between dietary docosahexaenoic acid and other long-chain polyunsaturated fatty acids on performance and fatty acid retention in post-smolt Atlantic salmon (Salmo salar). Fish Physiology and Biochemistry, 40 (4), pp. 1213-1227. https://doi.org/10.1007/s10695-014-9917-8
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Zhang Q, Xie D, Wang S, You C, Monroig O, Tocher DR & Li Y (2014) miR-17 is involved in the regulation of LC-PUFA biosynthesis in vertebrates: Effects on liver expression of a fatty acyl desaturase in the marine teleost Siganus canaliculatus. Biochimica et Biophysica Acta (BBA)- Molecular and Cell Biology of Lipids, 1841 (7), pp. 934-943. https://doi.org/10.1016/j.bbalip.2014.03.009
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Almaida P, Lucas-Sanchez A & Tocher DR (2014) Changes in mitochondrial membrane composition and oxidative status during rapid growth, maturation and aging in zebrafish, Danio rerio. Biochimica et Biophysica Acta (BBA)- Molecular and Cell Biology of Lipids, 1841 (7), pp. 1003-1011. https://doi.org/10.1016/j.bbalip.2014.04.004
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Fonseca-Madrigal J, Navarro JC, Hontoria F, Tocher DR, Martinez-Palacios CA & Monroig O (2014) Diversification of substrate specificities in teleostei Fads2: characterization of Δ4 and Δ6Δ5 desaturases of Chirostoma estor. Journal of Lipid Research, 55 (7), pp. 1408-1419. https://doi.org/10.1194/jlr.M049791
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Reis DB, Acosta NG, Almansa E, Navarro JC, Tocher DR, Monroig O, Andrade JP, Sykes AV & Rodriguez C (2014) In vivo metabolism of unsaturated fatty acids in Octopus vulgaris hatchlings determined by incubation with 14C-labelled fatty acids added directly to seawater as protein complexes. Aquaculture, 431, pp. 28-33. https://doi.org/10.1016/j.aquaculture.2014.05.016
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Murray DS, Hager H, Tocher DR & Kainz MJ (2014) Effect of partial replacement of dietary fish meal and oil by pumpkin kernel cake and rapeseed oil on fatty acid composition and metabolism in Arctic charr (Salvelinus alpinus). Aquaculture, 431, pp. 85-91. https://doi.org/10.1016/j.aquaculture.2014.03.039
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Martinez-Rubio L, Evensen O, Krasnov A, Jorgensen SM, Wadsworth S, Ruohonen K, Vecino JLG & Tocher DR (2014) Effects of functional feeds on the lipid composition, transcriptomic responses and pathology in heart of Atlantic salmon (Salmo salar L.) before and after experimental challenge with Piscine Myocarditis Virus (PMCV). BMC Genomics, 15, Art. No.: 462. https://doi.org/10.1186/1471-2164-15-462
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Betancor M, Howarth FJE, Glencross B & Tocher DR (2014) Influence of dietary docosahexaenoic acid in combination with other long-chain polyunsaturated fatty acids on expression of biosynthesis genes and phospholipid fatty acid compositions in tissues of post-smolt Atlantic salmon (Salmo salar). Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 172-173, pp. 74-89. https://doi.org/10.1016/j.cbpb.2014.04.007
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Xie D, Chen F, Lin S, Wang S, You C, Monroig O, Tocher DR & Li Y (2014) Cloning, Functional Characterization and Nutritional Regulation of Delta 6 Fatty Acyl Desaturase in the Herbivorous Euryhaline Teleost Scatophagus Argus. PLoS ONE, 9 (3), Art. No.: e90200. https://doi.org/10.1371/journal.pone.0090200