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Phagocytic activity of Limulus polyphemus amebocytes in vitro

Citation
Coates C, Whalley T & Nairn J (2012) Phagocytic activity of Limulus polyphemus amebocytes in vitro, Journal of Invertebrate Pathology, 111 (3), pp. 205-210.

Abstract
Phagocytosis of invading microorganisms is a fundamental component of innate immunity. The Atlantic horseshoe crab, Limulus polyphemus, possesses a single immune cell type, the granular amebocyte. Amebocytes release a repertoire of potent immune effectors in the presence of pathogens, and function in hemostasis. In contrast to other arthropod immunocytes, the properties of amebocyte phagocytosis remain poorly characterised, restricted by the technical challenges associated with handling these labile cells. We have addressed these challenges and observed the internalisation of microbial and synthetic targets by amebocytes in vitro. Confirmation of target internalisation was achieved using a combination of fluorescent quenching and lipophilic membrane probes: R18 and FM 1-43. Viability, morphological integrity and functionality of extracted amebocytes appeared to be retained in vitro. The phagocytic properties of L. polyphemus amebocytes described here, in the absence of endotoxin, are similar to those observed for arthropod immunocytes and mammalian neutrophils.

Keywords
Phagocytosis; Amebocytes; Limulus polyphemus; Cellular defence; Innate immunity; Fluorescent probes

StatusPublished
AuthorsCoates Christopher, Whalley Tim, Nairn Jacqueline
Publication date11/2012
PublisherElsevier
ISSN 0022-2011
LanguageEnglish

Journal
Journal of Invertebrate Pathology: Volume 111, Issue 3 (NOV 2012)

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