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Intraspecific diversity of Edwardsiella ictaluri isolates from diseased freshwater catfish, Pangasianodon hypophthalmus (Sauvage), cultured in the Mekong Delta, Vietnam

Citation
Bartie K, Austin FW, Diab A, Dickson C, Dung TT, Giacomini M & Crumlish M (2012) Intraspecific diversity of Edwardsiella ictaluri isolates from diseased freshwater catfish, Pangasianodon hypophthalmus (Sauvage), cultured in the Mekong Delta, Vietnam, Journal of Fish Diseases, 35 (9), pp. 671-682.

Abstract
A molecular epidemiology study was conducted on 90 Edwardsiella ictaluri isolates recovered from diseased farmed freshwater catfish, Pangasianodon hypophthalmus, cultured in the Mekong Delta, Vietnam. Thirteen isolates of E. ictaluri derived from diseased channel catfish, Ictalurus punctatus, cultured in the USA were included for comparison. All the E. ictaluri isolates tested were found to be biochemically indistinguishable. A repetitive (rep)-PCR using the single (GTG)5 primer was shown to possess limited discriminatory power, yielding two similar DNA profiles categorized as (GTG)5-PCR group 1 or 2 among the Vietnam isolates and (GTG)5-PCR group 1 within the USA isolates. Macrorestriction analysis identified 14 and 22 unique pulsotypes by XbaI and SpeI, respectively, among a subset of 59 E. ictaluri isolates. Numerical analysis of the combined macrorestriction profiles revealed three main groups: a distinct cluster formed exclusively of the USA isolates, and a major and minor cluster with outliers contained the Vietnam isolates. Antibiotic susceptibility and plasmid profiling supported the existence of the three groups. The results indicate that macrorestriction analysis may be regarded as a suitable typing method among the E. ictaluri species of limited intraspecific diversity. Furthermore, the findings suggest that E. ictaluri originating from Vietnam may constitute a distinct genetic group.

Keywords
Edwardsiella ictaluri; genotyping; Pangasianodon hypophthalmus; pulsed-field gel electrophoresis; Rep-PCR; Vietnam

StatusPublished
AuthorsBartie Kerry, Austin Frank W, Diab Amer, Dickson Cathryn, Dung Tu Thanh, Giacomini Mauro, Crumlish Margaret
Publication date09/2012
Date accepted by journal24/03/2012
PublisherWiley-Blackwell
ISSN 0140-7775
LanguageEnglish

Journal
Journal of Fish Diseases: Volume 35, Issue 9 (SEP 2012)

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