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First identification of Flavobacterium columnare infection in farmed freshwater striped catfish Pangasianodon hypophthalmus

Citation
Tien NT, Dung TT, Tuan NA & Crumlish M (2012) First identification of Flavobacterium columnare infection in farmed freshwater striped catfish Pangasianodon hypophthalmus, Diseases of Aquatic Organisms, 100 (1), pp. 83-88.

Abstract
The bacterium Flavobacterium columnare was recovered and identified as the aetiological agent causing freshwater columnaris infection in farmed striped catfish Pangasianodon hypophthalmus (Sauvage) fingerlings that had suffered high mortality rates within commercial hatchery ponds in Vietnam. The gross clinical signs were typical of columnaris-infected fish. Histological examination found numerous Gram-negative, filamentous bacteria present on the skin, muscle and gill tissues of affected fish. The yellow-pigmented bacteria were isolated and identified as F. columnare using primary, biochemical and PCR methods. An experimental immersion-challenge study with 2 strains was also performed. It fulfilled Koch's postulates and showed a median lethal concentration (LC50) of 4.27 × 105 and 1.66 × 106 cfu ml-1 for the F. columnare strains FC-HN and FC-CT, respectively. To the best of our knowledge this is the first report of freshwater columnaris infection in P. hypophthalmus.

Keywords
Flavobacterium columnare; White patch disease; Pangasianodon hypophthalmus

StatusPublished
AuthorsTien Nguyen Thi, Dung Tu Thanh, Tuan Nguyen Ahn, Crumlish Margaret
Publication date13/08/2012
PublisherInter-Research
ISSN 0177-5103
LanguageEnglish

Journal
Diseases of Aquatic Organisms: Volume 100, Issue 1 (AUG 13 2012)

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