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Article in Journal ()

Production of heterozygous and homozygous clones in Nile tilapia

Citation
Hussain MG, Penman D & McAndrew B (1998) Production of heterozygous and homozygous clones in Nile tilapia, Aquaculture International, 6 (3), pp. 197-205.

Abstract
Production of heterozygous and homozygous clones of Nile tilapia (Oreochromis niloticus L.) was successfully carried out. An outbred clonal line was produced by cross-breeding between a viable mitotic gynogenetic female and male (recessive mutation in a sex determining gene). At the same time, an inbred clonal line was produced by gynogenetic reproduction (retention of 2nd polar body) using optimal pressure/heat shock treatments. The heterozygous and homozygous nature of the two types of clonal lines were checked and identified at the adenosine deaminase ADA* marker locus. A model for the large scale production of similar clones is presented. The possible implications of such clonal lines in genetic research and in the culture of Oreochromis spp. are discussed.

Keywords
gynogenesis; heterozygous and homozygous clones; Nile tilapia (Oreochromis niloticus L.)

StatusPublished
AuthorsHussain M Gulam, Penman David, McAndrew Brendan
Publication date05/1998
PublisherSpringer
ISSN 0967-6120
LanguageEnglish

Journal
Aquaculture International: Volume 6, Issue 3 (MAY 1998)

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