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Article in Journal ()

Evolution and conservation of Characidium sex chromosomes

Citation
Utsunomia R, Scacchetti PC, Hermida M, Fernandez-Cebrian R, Taboada X, Fernandez C, Bekaert M, Mendes NJ, Robledo D, Mank JE, Taggart J, Oliveira C, Foresti F & Martinez P (2017) Evolution and conservation of Characidium sex chromosomes, Heredity, 119 (4), pp. 237-244.

Abstract
Fish species exhibit substantial variation in the degree of genetic differentiation between sex chromosome pairs, and therefore offer the opportunity to study the full range of sex chromosome evolution. We used restriction-site associated DNA sequencing (RAD-seq) to study the sex chromosomes of Characidium gomesi, a species with conspicuous heteromorphic ZW/ZZ sex chromosomes. We screened 9863 single-nucleotide polymorphisms (SNPs), corresponding to ~1 marker/100 kb distributed across the genome for sex-linked variation. With this data set, we identified 26 female-specific RAD loci, putatively located on the W chromosome, as well as 148 sex-associated SNPs showing significant differentiation (average FST=0.144) between males and females, and therefore in regions of more recent divergence between the Z and W chromosomes. In addition, we detected 25 RAD loci showing extreme heterozygote deficiency in females but which were in Hardy–Weinberg equilibrium in males, consistent with degeneration of the W chromosome and therefore female hemizygosity. We validated seven female-specific and two sex-associated markers in a larger sample of C. gomesi, of which three localised to the W chromosome, thereby providing useful markers for sexing wild samples. Validated markers were evaluated in other populations and species of the genus Characidium, this exploration suggesting a rapid turnover of W-specific repetitive elements. Together, our analyses point to a complex origin for the sex chromosome of C. gomesi and highlight the utility of RAD-seq for studying the composition and evolution of sex chromosomes systems in wild populations.

StatusPublished
AuthorsUtsunomia Ricardo, Scacchetti Priscilla C, Hermida Miguel, Fernandez-Cebrian Raquel, Taboada Xoana, Fernandez Carlos, Bekaert Michaël, Mendes Natalia J, Robledo Diego, Mank Judith E, Taggart John, Oliveira Claudio, Foresti Fausto, Martinez Paulino
Publication date10/2017
Publication date online26/07/2017
Date accepted by journal26/06/2017
PublisherSpringer Nature
ISSN 0018-067X
LanguageEnglish

Journal
Heredity: Volume 119, Issue 4 (2017)

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