Research output

Article in Journal ()

Resistance exercise initiates mechanistic target of rapamycin (mTOR) translocation and protein complex co-localisation in human skeletal muscle

Citation
Song Z, Moore DR, Hodson N, Ward C, Dent JR, O'Leary MF, Shaw A, Hamilton DL, Sarkar S, Gangloff Y, Hornberger TA, Spriet LL, Heigenhauser GJF & Philp A (2017) Resistance exercise initiates mechanistic target of rapamycin (mTOR) translocation and protein complex co-localisation in human skeletal muscle, Scientific Reports, 7, Art. No.: 14.

Abstract
The mechanistic target of rapamycin (mTOR) is a central mediator of protein synthesis in skeletal muscle. We utilized immunofluorescence approaches to study mTOR cellular distribution and protein-protein co-localisation in human skeletal muscle in the basal state as well as immediately, 1 and 3 h after an acute bout of resistance exercise in a fed (FED; 20 g Protein/40 g carbohydrate/1 g fat) or energy-free control (CON) state. mTOR and the lysosomal protein LAMP2 were highly co-localised in basal samples. Resistance exercise resulted in rapid translocation of mTOR/LAMP2 towards the cell membrane. Concurrently, resistance exercise led to the dissociation of TSC2 from Rheb and increased in the co-localisation of mTOR and Rheb post exercise in both FED and CON. In addition, mTOR co-localised with Eukaryotic translation initiation factor 3 subunit F (eIF3F) at the cell membrane post-exercise in both groups, with the response significantly greater at 1 h of recovery in the FED compared to CON. Collectively our data demonstrate that cellular trafficking of mTOR occurs in human muscle in response to an anabolic stimulus, events that appear to be primarily influenced by muscle contraction. The translocation and association of mTOR with positive regulators (i.e. Rheb and eIF3F) is consistent with an enhanced mRNA translational capacity after resistance exercise.

Keywords
cell biology; TOR signalling

StatusPublished
AuthorsSong Zhe, Moore Daniel R, Hodson Nathan, Ward Carl, Dent Jessica R, O'Leary Mary F, Shaw Andy, Hamilton David Lee, Sarkar Sovan, Gangloff Yann-Gael, Hornberger Troy A, Spriet Lawrence L, Heigenhauser George J F, Philp Andrew
Publication date10/07/2017
Publication date online10/07/2017
Date accepted by journal19/06/2017
PublisherSpringer Nature
ISSN No ISSN
LanguageEnglish

Journal
Scientific Reports: Volume 7 (2017)

© University of Stirling FK9 4LA Scotland UK • Telephone +44 1786 473171 • Scottish Charity No SC011159
My Portal