Article in Journal ()
Benitez-Dorta V, Caballero MJ, Betancor M, Manchado M, Tort L, Torrecillas S, Zamorano MJ, Izquierdo MS & Montero D (2017) Effects of thermal stress on the expression of glucocorticoid receptor complex linked genes in Senegalese sole (Solea senegalensis): Acute and adaptive stress responses, General and Comparative Endocrinology, 252, pp. 173-185.
The present study examined the short and mid-term effects of a rise in temperature from 18 ºC to 24 ºC on the expression of genes related to the stress response regulation in juveniles of Senegalese sole, Solea senegalensis. The animals were exposed to a temperature increase of 6 °C, after 1 month of acclimation at 18 ºC. After this process, samples of different tissues were collected from a total of 96 fish at four sampling points: 1 hour, 24 hours, 3 days and 1 week. The transcript levels of a set of genes involved in the stress response such as glucocorticoid receptors 1 and 2, corticotrophin-releasing factor, corticotrophin-releasing factor binding proteins, proopiomelanocortin A and B, and cellular stress defense (heat shock protein 70, 90AA and 90AB) were quantified at these sampling points. Additionally, blood samples were also taken to measure the circulating plasma cortisol concentration.
Cortisol; Temperature; Glucocorticoid receptor; Heat shock protein; Proopiomelanocortin; Corticotrophin-releasing factor; Corticotrophin-releasing factor binding proteins
|Authors||Benitez-Dorta Vanessa, Caballero Maria Jose, Betancor Monica, Manchado Manuel, Tort Lluis, Torrecillas Silvia, Zamorano Maria J, Izquierdo Marisol S, Montero Daniel|
|Publication date online||23/06/2017|
|Date accepted by journal||22/06/2017|
General and Comparative Endocrinology: Volume 252